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The BioAFM with Highest Resolution and Most Complete Biomechanics

The BioScope Resolve™ BioAFM provides the highest resolution imaging, most complete biomechanics capabilities, and fastest scanning of any bioAFM available. Specifically designed for integration onto inverted optical light and confocal microscopes, it enables investigation of a wide range of biological samples, from cells and tissues to molecular and protein structures. The New MIROView™ interface provides unique data sets by combining AFM biomechanics data with real-time confocal and fluorescence microscopy.

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The Highest Resolution Imaging of Any BioAFM — Any Time, Every Time



Double helix DNA — The unique mechanical stability and performance of the BioScope Resolve AFM on an optical microscope enables researchers to routinely resolve the major and minor grooves along the backbone of an individual DNA strand.




High-resolution cell imaging — With the BioScope Resolve and exclusive ScanAsyst®, high-resolution cell imaging has never been easier. This game-changing capability has allowed soft, flexible cell surface structures, such as microvilli, to be resolved for the first time on living cells by AFM.

Complete Cell Mechanics Data – Accurate, Repeatable

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PeakForce QNM images of Live MDCK Cells (Left: Height, Right: Modulus).

Widest range of ramp rates – At the core of quantitative nanomechanical maps are force-distance curves. Beyond characterizing elastic response, ramp rate dependent data provides insight into viscoelasticity or into molecular interaction times at the single molecule level. With our new FASTForce Volume covering rates from sub-Hz to 300 Hz, and PeakForce Tapping extending to 2 kHz, we provide the widest range of ramp rates for single points and imaging while maintaining pN force control.

Covering the whole spectrum — Our new RampScript™ allows the user to build, control and record complex nanomechanical measurements for use in protein pulling, ligand-receptor interaction, cell relaxation and viscoelastic probing. Its low force trigger capability, and latency-free implementation build on the system’s core performance to provide the most accurate script execution and force control. Ramping scripts are created in an intuitive drag and drop process and provide ultimate flexibility including per segment optical synchronization.

Ultimate force control —  PeakForce QNM®,has become the preferred mode for nanomechanical mapping. With new algorithms, it delivers highest resolution property maps at pN forces on live cells. Together with “No Touch” tip calibration, PeakForce QNM delivers the fastest quantitative mechanical characterization of live cells, providing a complete force curve for every pixel at actuation rates from 125 Hz to 2 kHz.

Investigating Cell Dynamics – Without Compromise

Sample courtesy of Dr. Ivan Liashkovich and Dr. Hermann Schillers, University of Münster, Germany

Fast Tapping on living cells - Bioscope Resolve is the only BioAFM that enables fast scanning while simultaneously providing the large piezo scanner range necessary for capturing the dynamic behavior of living cells in real time (XYZ = 100µm x 100µm x 15µm). The Fast Tapping capabilities of Resolve are truly a step forward in conducting high-resolution AFM studies at timescales relevant to cellular processes. Together with the integration of advanced optical microscopy and environmental control, live cell studies have never been easier.

Dynamics of cell migration – High-resolution Fast Tapping movies enable direct observation of membrane ruffling events and associated dynamic rearrangement of the actin cytoskeleton at the leading edge of a living endothelial cell. Images were obtained at 35 seconds/frame (left – 37µm scan size) and 12.5 seconds/frame (right - 27µm scan size).

Combined Fluorescence and Biomechanics – Seamless, Simple

Expanded measurement possibilities — The new MIROView GUI ensures expert data generation via fully synchronized AFM images, force maps, and single-force curves with optical images and data. 

Great optical integration — BioScope Resolve AFM works seamlessly with an optical microscope, thanks to communication protocols developed with researchers’ applications in mind. Pixel by pixel, line by line, and image by image, optical synchronization delivers flexibility never seen before on an AFM.

 The perfect environment — With a new acoustic hood, work station, and a complete suite of life science research accessories, such as a sample heater, perfusion cell, and a top-down camera, the BioScope Resolve provides all your bioAFM research needs.



Unparalleled Optical/AFM Integration

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For years, AFM systems placed on optical microscopes forced the user to work around the AFM, not with the AFM. The BioScope Resolve revolutionizes the way the AFM works and communicates with the optical microscope by synchronizing between them.

This integration ensures that you get superior BioAFM data without sacrificing your optical image quality. We know how important your optical image is, which is why BioScope Resolve was designed to offer absolute optical and AFM integration without sacrificing image quality.  

Cell Probing with FluidFM® Integration

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BioScope Resolve was designed as a platform for probing individual living cells. The integration of Cytosurge’s FluidFM® now expands the possibilities for conducting new types of single cell studies. Where PeakForce QNM has provided the pN force control for recognition mapping and studies of receptor binding kinetics, FluidFM addresses probe functionalization by permitting quick exchange of attached colloids in situ through fluid control. FluidFM also enables injection or manipulation of individual cells. Using Fast Tapping, PeakForce QNM, and Resolve’s Advanced Correlation stage, subsequent cell changes can be tracked on dozens of cells. Or FluidFM can be used in conjunction with Resolve’s optical synchronization to correlate AFM adhesion and confocal fluorescence data in investigations of bacterial biofilms and pathogenic pathways. (FluidFM probe shown here.)