FastScan Bio Videos

Capturing Biological Dynamics with Ease

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DNA origami on mica surface

Demonstrated for the first time with an atomic force microscope, Bruker’s FastScan Bio AFM enabled observation of free origami molecule nucleation in solution as triggered by the origami molecule concentration.

Sample provided by:
Michael L. Norton and Masudur Rahman
Department of Chemistry
Marshall University, WV, USA 

 

 

Stem Cell Migration

C2C12 stem cells migrating on glass surface
Seen here is phase data of lamellopodia extending through polymerization of the Actin Cytoskeleton in the direction of motion. The cell membrane then relieves resulting tension by sliding along. Time: 1hr. AFM: FastScan with FastScan-Bio cantilevers. Sample courtesy of R. Medda, E. Schwab and E.A. Cavalcanti-Adam; MPI for Intelligent Systems - New Materials and Biosystems

E. coli Time-Lapse Videos - CM15 Antimicrobial Activity on the Nanoscale Structure of E. coli Bacterial Outer Membrane

The dynamics of CM15 antimicrobial activity on the native outer membrane of live E. coli cells was observed by high speed AFM with nanometer resolution. The movie was recorded at 8 seconds/frame with each frame captured at a scan size of 300nm with a resolution of 1024x256 pixels. High-resolution imaging of the outer membrane surface of individual live E. coli cells revealed the presence of nanometer-sized membrane-associated features that exhibited an ordered, lattice-like structure. These structures are similar to those previously observed on excised patches of bacterial outer membrane that were found to be closely packed porin molecules. CM15 was introduced into the imaging fluid during continuous imaging at a final concentration of 20ug/mL, as indicated by the inset graph (Membrane Surface Roughness vs. Time). At ~96s after the addition of CM15, the cell membrane is observed to increase in roughness accompanied by the disappearance of the ordered membrane surface structures. Membrane protrusions start to form, consistent with micellization, followed by the appearance of "pore-like" lesions ~15nm in diameter. Once the effects of CM15 were initially observed, the subsequent changes in the cell surface occurred fairly rapidly over ~224s, at which point imaging of the cell surface becomes unstable.

E. coli Time-Lapse Videos - CM15 antimicrobial activity on live E. coli cells

The dynamics of CM15 antimicrobial activity on live E. coli cells was observed by high speed AFM at 18 seconds/frame, with each frame captured at a scan size of 3um at a resolution of 1024x256 pixels. CM15 was introduced into the imaging fluid during continuous imaging at a final concentration of 10ug/mL, as indicated. The initial stages of CM15 activity were observed as an increase in the roughness of the bacterial cell membrane with the onset of these changes occurring at different time points for individual cells, as highlighted in the movie. Changes to the surface of bacterium A were first observed at ~7.5min. Bacteria B and C, however, do not reveal any changes to their membrane surface until ~14min and ~17min, respectively. Phase images are shown as the high contrast allows us to clearly see changes in the surface of the cells

FastScan Bio Lipid Bilayer Time Series

Consecutive images recorded at 0.16 frames/sec demonstrate the stability of the taller DPPC gel phase domains and hence the highly sensitive force control of the Dimension FastScanTM at high scan speeds. Imaging was conducted using TappingModeTM under aqueous conditions. Height images are shown. (Image size = 3um; Z-scale = 3.2nm)