Proteomics - Protein analysis: identification, quantitation and characterization

The last two decades have seen significant advances in technology and new methodologies which have made proteomics an extremely powerful tool for protein scientists, biologists and clinical researchers.
The introduction of the timsTOF Pro™ coupled with the revolutionary PASEF™ acquisition mode is breaking new boundaries in speed, sensitivity, selectivity and robustness, allowing scientists to routinely explore parts of the proteome not previously accessible.

The direct analysis of proteins from a tissue or biofluid raises a variety of analytical challenges. Protein expression varies depending on the genetic background of an individual, but also on time, localization, and as a physiological response to external stimuli (stress, disease, aging, effort etc.). Moreover, because of the combined effects of alternative splicing, point mutation, post-translational modifications and endogenous proteolysis a given protein (gene expression product) can be expressed as many different proteoforms, each having a dedicated biological activity.

Fully addressing that complexity can only be achieved using orthogonal approaches. Bruker offers a dedicated panel of solutions to deal with these challenges.

The timsTOF Pro powered by PASEF for the first time enables fast, ultra-sensitive shotgun proteomics and relative protein quantitation at high throughput over large cohort and low abundance sample studies, at an unprecedented level of robustness.

In-depth PTM (Phospho, Glyco etc.) profiling and protein characterization that take advantage of the unique peak capacity that results from the coupling of TIMS to a Q-TOF. In depth ESI-based PTM (Phospho, Glyco, etc.) profiling and protein characterization take advantage of the unique peak capacity which results from TIMS technology coupled to a Q-TOF. The unique fragmentation patterns resulting from an LC-MALDI TOF-TOF analysis enables in-depth Post Traductional Modifications (Phospho, Glyco, ect)profiling and extensive protein characterization.

Proteoform Profiling and characterization solutions take advantage of Bruker’s UHR-Q-TOF’s unique capacity to analyze complex mixtures of intact proteins to detect proteoform patterns that are lost by the standard bottom-up approach.

On-tissue peptide and proteoform profiling to add the spatial localization information.