With new advances in field of view, sensitivity, wavelength, and sample accommodation, the Ultima 2Pplus delivers an ideal combination of flexibility, resolution, imaging depth and speed, allowing users to perform simultaneous imaging, stimulation and electrophysiology protocols with greater efficiency and effectivity. The system is designed specifically for intravital imaging, with fully motorized control of the objective X-Y-Z position, as well as two axes of rotation for precise imaging orientation. A second scan path enables simultaneous imaging and photoactivation. The system’s optimized optical train confers exceptional performance to the very edges of the large field.
The Ultima 2Pplus field of view is over 50% larger to facilitate modern optogenetics experiments, which require simultaneous imaging and photostimulation of distant regions in a sample. A new, custom optical design preserves spatial and temporal resolution throughout the entire field, which is essential for researchers to be able to continue their work without changing experimental designs.
The Ultima 2Pplus light collection path ensures detection of almost all photons scattered from dim, thick and opaque biological samples. The superior collection efficiency is provided with 2-inch or larger optics
throughout the emission path up to the sensitive close-proximity detectors. As two-photon microscopy has become well adopted, emerging three-photon microscopy offers the potential to image even deeper into
samples. Since further infrared wavelengths are scattered even less, three-photon excitation has the promise to be able to image several millimeters into the brain. Ultima 2Pplus already uniquely incorporates this capability to take your research to new levels of potential.
The Ultima 2Pplus empowers sophisticated optogenetics experiments with Bruker's Neuralight 3DTM Spatial Light Modulator option. Large populations of targets can be stimulated simultaneously in multiple focal planes with computer-generated holograms. During 3D holographic experiments, targets at different depths are imaged with a custom-designed, optically-corrected ETL focusing module that allows for independent Z-positioning of the imaging plane relative to photostimulation.
|Scanning Method||Matched pair of 6 mm Cambridge galvanometers with raster and spiral scanning capabilities|
|Field of View||~1.404 mm x 1.404 mm with 16x objective (up to 28.3 mm FN)|
|Scan Speed||Raster scan: 1.65 fps at 512 x 512, >12 fps at 64 x 64
Spiral scan: 6 fps at 512 x 512, ~30 fps at 64 x 64
|Scan Customization||User-definable straight, freehand and circular (infinite) linescan with included software; user-definable pixels per line and lines per scan from 1 - 2048; up to 128x scan zoom; 360° of scan rotation; point scan|
|Uncaging Option||Second set of matched 3 mm or 6 mm Cambridge galvanometers in the same scan head to provide high-precision visible or multiphoton laser sample photomanipulation|
|High-Speed Imaging Option||8 kHz resonant galvanometer; 30 fps at 512 x 512, >1300 fps at 512 x 8 region of interest|
|Reflected Non-Descanned||1 - 4 hand-picked Hamamatsu Multi-Alkali PMTs; upgradeable to high-sensitivity Hamamatsu GaAsP PMTs|
|Transmitted Non-Descanned||1 - 2 hand-picked Hamamatsu Multi-Alkali PMTs; upgradeable to high-sensitivity Hamamatsu GaAsP PMTs|
|Dodt||Single Hamamatsu PMT for DIC-like image collection|
|Transmitted||Single Hamamatsu PMT for transmitted light image collection|
|Camera||Standard C-mount camera port built into scan head|
|Multiphoton Laser||Optimized for multiphoton laser input from 690 - 1700 nm|
|Epifluorescence||High-Powered LED in epifluorescence turret|
|Visible Laser||Fibered laser inputs for visible laser introduction|
|Ultima Laser Rating||Class 1 (contains Class 3b and Class 4 lasers) with light box|
|Helios Laser Rating||Class 3b|
|LED||Full-field photoactivation with LED module|
|Bruker X, Y Stage||Variable height with ~15 cm X and ~7.5 cm Y movement and ~0.3 μm step size|
|Bruker X, Y Microscope Base||Fine and coarse-movement platform for scope with ~35 mm travel and 0.1 μm step size|
|Bruker Z-Focus||Range of 30 mm with ~0.2 μm step size|
|Bruker Z-Piezo||Travel range of up to 1000 μm with 0.05 μm step size|
|Bruker Orbital Nosepiece||Motorized control of objective angle and rotation|
|Prairie View Imaging||Software fully integrated with scan head for easy imaging; customizable scan settings for optimization of specimen excitation; integrated control of laser power and PMT high voltages|
|Z-Series||Easy creation of depth stacks with user-customizable slice number, step size and laser power|
|T-Series||Easy creation of complex series involving Z-Series and triggered images|
|Stage Montage||Atlas Imaging simplifies setup and optimizes acquisition of 2D and 3D stage montages|
|Peripherals Integration||Wavelength and power control available for multiphoton and visible laser launches|
|Photoactivation||User-defined points and regions with synchronized laser modulation|
|Regions of Interest||User-defined regions for faster scanning capabilities|
|Brightness Over Time||Intensity mapping and plotting for user-defined regions over time|
|Voltage Inputs/Outputs||Signal inputs and outputs for electrophysiological experiments, stimulus control, and synchronization with external devices|
We appreciate the modular nature of the Ultima hardware and software. The systems now available range from basic imaging systems to the dual-laser photo-stimulation multi-mode imaging workstation. We have continued updating many of our 14 machines, including our ten year old systems. The Team Viewer connection and support is a huge asset for novice owners and for significant software changes.