E-Book: Super-Resolution Microscopy
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Light microscopy offers many advantages for the investigation of biological samples. However, traditional methods are limited by diffraction to a resolution of a few hundred nanometers. Due to this limitation, these methods cannot discern many intracellular structures and activities of critical importance to contemporary life science research.
Super-resolution techniques can surpass the diffraction limit to enable imaging of specifically labeled molecules well beyond the optical diffraction limit, down to 20 nm laterally. With this enhanced resolving power, super-resolution microscopy methods allow the imaging and quantitative analysis of cellular dynamics and nanostructures that were previously inaccessible. As a result, super-resolution microscopy overcomes some key challenges associated with nanoscale biological imaging.
This guide focuses primarily on the single-molecule localization microscopy (SMLM) method of super-resolution microscopy.
Readers can expect to learn more about:
- The principles and advantages of super-resolution micrscopy for life sciences research;
- Super-Resolution Microscopy applications across life sciences disciplines (e.g., neuroscience, cell biology, genomics, and virology);
- The common approaches to achieving beyond diffraction-limited imaging, including localization and labeling techniques;
- Recent and cutting-edge super-resolution microscopy-related advancements (e.g., biplane technology, top-hat illumination, and microfluidics); and
- Future trends in super-resolution microscopy research.
