Disulfide bond analysis with DisulfideDetect
The tertiary structure of therapeutic proteins is key to their activity and stability. Due to the complexity of these proteins and the fact that they will contain multiple disulfide bonds, analysis is a challenge, often requiring several LC/MS runs with the tryptic digests from reduced and non-reduced protein and a manual comparison of these two analyses.
The DisulfideDetect workflow allows the fully automated analysis of disulfide bonds in biopharmaceuticals, based on a single digest of the unreduced protein and without prior knowledge of enzyme specificity or native disulfide bonds. The workflow also provides a semi-quantitative scrambling analysis as well.
DisulfideDetect software sets a new standard in ease-of-use for detecting and evaluating disulfide bonds within therapeutic proteins, such as monoclonal antibodies. It provides an easy to understand, visually-based, workspace for disulfide bond analysis for both native and non-native bonds as well as inter- and intra-chain disulfide bonds within the protein/antibody.
DisulfideDetect software uses established methods from existing LC-MALDI instruments like the UltrafleXtremeTM, so if your lab is utilizing LC-MALDI you're all set! Just add the DisulfideDetect Module to begin analyzing disulfide bonds in your biotherapeutics. No additional hardware is necessary.