Extremely sensitive analytical methods are required for accurate low-level identification, and quantitation of zero-tolerance compounds in food. The broad-spectrum antibiotic Chloramphenicol (CAP) is such a compound. Determination of CAP is often performed by LC-MS/MS using MRM measurements on traditional triple-quadrupole instruments. Using the ionBooster, CAP can be determined in an LC-MS full scan method for lower limit quantification and confirmation on a maXis UHR-TOF system, without compromising other analytical information.
The ionBooster achieves a Limit of Quantitation (LOQ) more than 100 times lower than the minimum required performance level (MRPL).
Top: Compare Chloramphenicol signal trace of ionBooster with traditional Electrospray at 0.25 μg/kg in matrix (CAP in beef muscle).
Bottom: Six replicate injections, at 0.0025 μg/kg CAP in matrix meat. Mean Signal: Noise 10:1, Peak width 3.4 sec (FWHM), Peak area RSD 9%. Figures from Application Note ET-27.