DeutEx Software for HDX‑MS Data Analysis

Efficiency & Insight for HDX-MS data

DeutEx: Speed and precision for HDX-MS analysis

Processing speed

DeutEx calculates deuteration levels for all peptides and conditions in no time, enabling quick turnaround for complex datasets.

Confidence

Interactive curation ensures flexibility and reliability in your results.

Data Compatibility

Supports Bruker TIMS, MRMS, and QTOF instruments.

Data visualization

Full flexibility by built-in visualization tools.

Complete HDX-MS Workflow Integration

DeutEx seamlessly integrates with Bruker’s LC-MS/MS solutions by offering an efficient interface for the processing of HDX data and automated calculation of deuteration levels across time points and replicates

Retention time-based filtering and extraction of multiple charge states
Compatible with protein modifications and fully compliant with Unimod syntax
Interactive review and manual data curation
Export options aligned with HDX-MS reporting standards
Visualization tools for structural and dynamic protein analysis including statistics

Streamlined peptide mapping based on protein search engine and on measured non-deuterated sample.

Save Time, Gain Confidence in your HDX Experiments

All results window depicting deuteration rates for all assigned peptides and conditions/time points measured.

DeutEx eliminates bottlenecks in HDX-MS data processing. Researchers benefit from:

Faster turnaround for complex datasets
Designed for multi-condition HDX experiments
Reliable and reproducible results
Compliance with HDX-MS best practices for publication
Data visualization including statistics
Ease of use

From Raw Data to Intuitive Insights: Automated HDX MS Visualization at Every Level

Fully automated visualization tools that transform HDX-MS results into clear, interpretable figures with integrated statistical filtering:

Coverage maps
Rainbow or differential heat maps
Butterfly or Woods plots
Direct integration with PyMol for 3D structure coloring

Coverage map of apo-myoglobin visualized within DeutEx. For rapid assessment of peptide coverage across the protein sequence.

Butterfly plot of holo- and apo-myoglobin generated by DeutEx. For intuitive comparison of deuterium uptake across experiments.

DeutEx supports your HDX workflows

DeutEx empowers research in:

Drug Design & Development: Identify binding sites and optimize drug candidates
Biopharmaceuticals: Ensure stability and efficacy of therapeutic antibodies
Structural Biology: Complement X-ray, cryo-EM, and NMR studies
Disease Research: Investigate protein mechanisms and therapeutic targets

3D structure coloring based on rainbow heatmap (apo-Mb) and differential heatmap (holo- versus apo-Mb). To highlight structural dynamics and condition-dependent changes.

"DeutEx has been a very intuitive software to use. The data processing workflow is easy to understand and any changes to the settings or data used for the calculations (e.g. adjusting isotopic distribution or retention time range) are reprocessed very quickly."

Liliana Pedro, Ph.D., Senior Scientist, Orion Pharma, Espoo, Finland

"We like it that besides the deuterium uptake plots, we can also visualize envelop widths and peptide log intensities across the different time points, making it easier to control for outliers and errors."

Kai Sinervo, Senior Research Scientist, Orion Pharma, Espoo, Finland