PaSER 2022

Break the data analysis bottleneck by integrating real-time database search and smart acquisition provided by PaSER

Universally available

• All current and future timsTOF Pro and fleX platforms are PaSER accessible
  

4D-Proteomics™

• CCS-aware data is rich, even so real-time database search capabilities are a reality with PaSER including with PTMs like phosphorylation

Massive parallelization with GPU power

• PaSER is GPU powered, provides massive compute power across thousands of CUDA cores

PaSER Data Review

• View the intricate details of your data from the high level experimental information to a specific fragment ion spectrum of interest with confidence from the integrated viewer onboard of all PaSER boxes

• PaSER is smart, where a user defined qualification of proteins or peptides at the end of a sample acquisition determines the progression of your sample queue, this checks suitability, save precious samples, expensive consumables and instrument time
  

• No more worrying about injecting those precious samples.
  
• No more worrying of wasted instrument time.
  
• No more worrying about those samples with expensive reagents and sample prep, only to inject on a poor performing system. 
  
• No more waiting for the QC runs to determine system suitability.
  
• No more waiting for search results during method development.
  
  

Blazing search speed with uncompromised search results.

A) Protein ID from replicate DDA runs of a human cell lysate using PaSER for real-time results and benchmarked against MaxQuant

B) Peptide ID from replicate DDA runs of a human cell lysate using PaSER for real-time results and benchmarked against MaxQuant

C) PaSER and MaxQuant identify 97% of the same proteins and nearly 90% of the same peptides.

PaSER provides consistent results obtained from the real-time search, identical to offline search of the same data. Since the database search algorithm is run on the GPU, the search times are negligible compared to CPU based searches.

In addition to real-time search the opportunity to search a larger space for specific applications, including PTM studies, becomes reality.

MOMA viewer powered by TIMS Viz provides the powerful ability to view 4D-Proteomics data like never seen before. The mobility separation and focusing upstream from the quadrapole and TOF results in the separation of isobaric and near isobaric species. Using other technologies and in the case of PTM enrichments and the analysis of complex samples it is common that co-eluting species that are isobaric or near isobaric in mass co-fragment. The timsTOF Pro/fleX obviates this by first separating ions by their collisional cross section. The ability to visualize, search and sort on such events has never been available before. We term isobaric masses with different mobility values mobility offset mass aligned (MOMA), and TIMS Viz can tease such events out within complex runs. TIMS Viz allows you to set tolerances to your discretion while simultaneously overlaying both features and PSMs that were observed post database search. TIMS Viz enables you to view what you never knew before.