Real-time results = efficiency
Break the data analysis bottleneck by integrating real-time database search and smart acquisition provided by PaSER
Massive parallelization with GPU power
PaSER Data Review
Blazing search speed with uncompromised search results.
A) Protein ID from replicate DDA runs of a human cell lysate using PaSER for real-time results and benchmarked against MaxQuant
B) Peptide ID from replicate DDA runs of a human cell lysate using PaSER for real-time results and benchmarked against MaxQuant
C) PaSER and MaxQuant identify 97% of the same proteins and nearly 90% of the same peptides.
PaSER provides consistent results obtained from the real-time search, identical to offline search of the same data. Since the database search algorithm is run on the GPU, the search times are negligible compared to CPU based searches.
In addition to real-time search the opportunity to search a larger space for specific applications, including PTM studies, becomes reality.
MOMA viewer powered by TIMS Viz provides the powerful ability to view 4D-Proteomics data like never seen before. The mobility separation and focusing upstream from the quadrapole and TOF results in the separation of isobaric and near isobaric species. Using other technologies and in the case of PTM enrichments and the analysis of complex samples it is common that co-eluting species that are isobaric or near isobaric in mass co-fragment. The timsTOF Pro/fleX obviates this by first separating ions by their collisional cross section. The ability to visualize, search and sort on such events has never been available before. We term isobaric masses with different mobility values mobility offset mass aligned (MOMA), and TIMS Viz can tease such events out within complex runs. TIMS Viz allows you to set tolerances to your discretion while simultaneously overlaying both features and PSMs that were observed post database search. TIMS Viz enables you to view what you never knew before.
“Real-time results delivered by the PaSER box on our timsTOF Pro has been a huge time saver. This allows us to develop methods in real-time, inform on LC and instrument performance and overall a major gain in efficiency.“
“Innovative software tools are a necessity to address unanswered biological questions with mass spectrometry. The trapped ion mobility functionality and the robustness of the timsTOF Pro offer unique bottom-up proteomics capabilities that can be effectively used to study many diseases.”
For Research Use Only. Not for use in clinical diagnostic procedures.