SkyScan 2214

3D X-ray Microscopy at the Nanoscale

Ultimate Performance at your Command

herostageskyscan2214

Highlights

Highlights

300 x 400 mm
largest size that can be accommodated
Whether the sample is enormous or small, the SKYSCAN 2214 has the flexibility to image them all.
160 keV
penetrating power
Quick change filament for switching between W for highest penetration or LaB₆ for highest resolution
<500 nm
true 3D spatial resolution
There are many ways to define resolution, but the most important is the ability to resolve 2 features in the resulting 3D model.

SKYSCAN 2214 – Nanoscale X-ray Microscopy

The new MULTISCALE X-ray nanotomograph SKYSCAN 2214 covers the widest range of object sizes and spatial resolutions in a single instrument. It opens unique possibilities for 3D imaging and exact modeling of all types of biological samples, including mineralized tissues, soft tissues, plants, insects, paleontological samples, etc.

 

The instrument allows scanning and 3D non-destructive reconstruction of the internal microstructure of objects as large as 300mm in diameter as well as submicron resolution for small samples.

 

The system contains an "open type" transmission X-ray source with <0.5 microns spot size and a diamond window. It can be equipped with up to four different X-ray detectors for maximum flexibility: flat-panel for large objects, 11Mp cooled CCD with wide field of view, 11Mp cooled CCD with medium field of view, and 8Mp cooled CCD for highest spatial resolution. Automatic variable acquisition geometry and phase-contrast enhancement allows best possible quality in relatively short scanning time.

 

The SKYSCAN 2214 is complemented by 3D.SUITE. This comprehensive software suite covers GPU-accelerated reconstruction, 2D/ 3D morphological analysis, as well as surface and volume rendering visualization

SKYSCAN 2214 walk-up video

What is XRM? video

Eigenschaften

Key Features

SKYSCAN 2214 Features

Source

The SKYSCAN 2214 uses a latest generation open-type X-ray source. The source offers true spatial resolution below 500 nm, an X-ray energy up to 160 keV and source power up to 16 W. The source is practically maintenance-free with an extremely easy pre-aligned filament replacement procedure.

The SKYSCAN 2214 has an open-type (pumped) nanofocus X-ray source with diamond window. It produces an X-ray beam with peak energy from 20 kV to 160 keV and is supplied with two types of cathodes. The tungsten (W) cathodes operate in the full range of accelerating voltages up to 160 kV and provide a spot size down to 800 nm. The lanthanum hexaboride (LaB6) cathodes can be used for accelerating voltages from 20 kV to 100 kV and provide a spot size of the X-ray beam smaller than 500 nm to achieve the highest resolution in imaging and 3D reconstruction. The JIMA resolution pattern indicates that 500 nm structures can be easily resolved.

For long-term stability of the focal spot size and position of the emission point, the X-ray source is equipped with a liquid cooling system which contains a re-circulator providing precise temperature stability of the cooling fluid.

SKYSCAN 2214 Features

Detectors

The SKYSCAN 2214 can be equipped with up to four X-ray cameras for ultimate flexibility: three CCD cameras with different resolution and field of view and one large-area flat panel detector. All cameras can be selected with a single mouse click. The different CCD cameras can be retro-fitted at any point of time during the system’s lifetime.

All three CCDs can take images in the central beam position and in two offset positions to double the field of view. The images in the two offset positions are automatically stitched together with compensation of the shifts and possible intensity differences.

Using CCD detectors with small pixel size allows extension of high-resolution imaging and 3D reconstruction to large objects. The built-in detector flexibility enables adjusting the field of view and spatial resolution according to the object size and density. An advanced reconstruction from a volume of interest provides scanning of a selected part of a large object with high resolution without compromising image quality.

Additionally, the field of view can be increased horizontally and vertically by using offset camera positions and vertical object movement.

SKYSCAN 2214 Features

In-situ stages

The high-precision object stage of the SKYSCAN 2214 supports objects up to 300 mm diameter and 20 kg in weight. The air-bearing rotation motor allows precise rotation of objects at very high accuracy, and the integrated micro-positioning stage guarantees a perfect sample alignment.
The SKYSCAN 2214 has a large and easily accessible sample chamber to allow scanning of big objects as well as mounting of optional stages. On top plenty of space is available for peripheral equipment.

The Bruker material testing stages are designed to perform compression experiments up to 4400 N and tensile experiments up to 440 N. All stages automatically communicate through the system’s rotation stage, without the need of any cable connections. Using the supplied software, scheduled scanning experiments can be set up.

Bruker's heating & cooling stages can reach temperatures of up to +80 °C or 30 °C below ambient temperature. Just like the other stages, no extra connections are needed, and there is an automatic recognition of the stage. Using the heating & cooling stages, samples can be examined under non-ambient conditions, to evaluate the effect of temperature on the sample’s microstructure.

The SKYSCAN 2214 is fully compatible with stages from DEBEN. With the included adapter, the DEBEN stage can be simply placed onto the rotation stage of the SKYSCAN 2214.

Cooling Stage
Material Testing Stage
Deben Stage

Anwendungen

Highlighted Applications

Mineralized Tissues

The SKYSCAN 2214 provides ultimate nanoCT imaging performance without compromise. Four cameras are fitted – one large format 6 megapixel cMOS flat panel for imaging up to high voltages and large skeletal or dental samples, and a range of three small format detectors for the optimal choice of resolution and x-ray energy window for every conceivable bone sample scan. 

Submicron voxel scans provide crystal clear resolution of bone osteocyte lacunae and micro-scale mineralization structure, as well as architecture of biomaterial scaffolds at nano-scale resolution.

Phase retrieval (Paganin) provides a new dimension of analysis of hitherto little studied bone micro-mineralization patterns.

Orthopedic research with sheep, primate or similar models is handled with the 14cm scan field of view and 160kV x-ray source. Realize your orthopedic and biomechanical research goals with mechanical testing and temperature control stages.

Morphometry with comprehensive 3D and 2D parameters, with densitometry including BMD calibration references over the preclinical size range. Advanced 3D image analysis functions include 3D registration, adaptive thresholding, Euler connectivity, fractal, anisotropy and stereology, filtering, Boolean logical operators and much more.

Soft Tissues

Ex vivo nano-CT scanning of biological tissues, with sub-micron pixel size, is like histology or electron microscopy but in true, deep 3D - a fantastic method to show internal structures non-destructively. Contrast agents or chemical drying can improve the image quality by further enhancing or differentiating tissue densities. The SKYSCAN 2214 is playing a leading role in the emergence of a new imaging discipline – microCT histology and histomorphometry.

The unmatched versatility of this system ensures that each sample will be scanned with optimized parameters and the best resolution and contrast. 

Four cameras are fitted – a large format 6 megapixel flat panel for imaging up to high voltages and large tissue samples, and a range of three small format cameras for the optimal choice of resolution and x-ray energy window for every conceivable sample scan.

Comprehensive 3D image analysis capability including morphometry and densitometry, 3D registration, segmentation and advanced image processing methods.

Plant and Animal Biology

MicroCT is exceptionally good for the visualization of the finest details of internal biological structures. This imaging method creates a nano-scale 3D x-ray attenuation map without harming or destroying the scanned object. Practically all biological tissues can be visualized and analyzed with little or no need of special sample treatment. Submicron voxel resolution allows imaging of small insect and plant or seed structures with powerful magnification and rich detail.

The space in the scanning chamber and the precision of the rotation stage make sure that all types of samples can be scanned, from the small zebrafish and preserved zoological and botanic samples to potted plants and rock-embedded fossils.

Four cameras are fitted – a large format 6 megapixel flat panel for imaging up to high voltages and large samples, and a range of three small format cameras for the optimal choice of resolution and x-ray energy window for every conceivable biological sample scan.

The software suite enables morphometry with comprehensive 3D and 2D parameters, and advanced 3D image analysis functions include 3D registration, adaptive thresholding, Euler connectivity, fractal, anisotropy and stereology, filtering, Boolean logical operators and much more. 

Spezifikationen

SKYSCAN 2214 Specifications

Feature

Specification

Benefit

 X-ray source

20-160 kV

16 W max.

User exchangeable filament

Optimize for max power (W) or max resolution (LaB6)

Rotatable diamond window for maximum lifetime

X-ray detector

6 Mp active pixel flat-panel

11 Mp large format cooled CCD

11 Mp mid format cooled CCD

8 Mp hi-res cooled CCD

Variety of pixel and detector sizes allow balance between detector resolution, coverage and counting statistics

Available with 1, 2, 3 or 4 detectors

Field upgradable to add detectors

Image Formats

Up to 8000 x 8000 x 2300 pixels after a single scan

User selectable image size allows balance of dataset size and needed resolution

Software allows downsizing after data collection

Resolution

60 nm smallest pixel size
<500 nm low-contrast resolution (10% MTF)

Simple graphics control for optimizing experiment resolution based on selected detector, sample and detector distance

Adjustable source focus size to balance maximum power and resolution

Positioning Accuracy

<50 nm for rotation
Anti-vibration granite platform with pneumatic leveling

Air bearing sample stage provides smooth rotation

Simple chuck style mounting of sample posts

Mechanical and electrical interface for advanced materials research stages

Maximum Object Size

300 mm in diameter (140 mm scanning size)

400 mm in length

Maximum object weight 20 kg

Both the power and room to scan large samples

Precise positioning of small samples near the source to maximize magnification

Dimensions W 1800 mm x D 950 mm x H 1680 mm

Weight: 1500 kg

Efficiently designed to optimize use of lab space

Source maintenance access via interlocked large sliding door

Software

POSITION, SCAN, RECONSTRUCT and ANALYZE

Bruker XRM solutions include all software needed to collect and analyze data. An intuitive graphical user interface with user guided parameter optimization support both expert and novice users. By using the latest GPU powered algorithms, reconstruction time is substantially reduced. CTVOX, CTAN and CTVOL combine to form a powerful suite of software for both qualitative and quantitative analysis of models.

Measurement Software:
SKYSCAN 1272 – Instrument control, measurement planning and collection

Reconstruction Software:
NRECON – Transforms the 2D projection images into 3D volumes

Analysis Software:
DATAVIEWER – Slice-by-slice inspection of 3D volumes and 2D/3D image registration

CTVOX – Realistic visualization by volume rendering

CTAN – 2D/3D image analysis & processing

CTVOL – Visualization of surface models to export for CAD or 3D printing

Support

SKYSCAN 2214 - Service & Support

Available services include

  • Help desk support from highly-skilled troubleshooting professionals, to isolate and resolve hard- and software problems
  • Web-based remote instrument service for service diagnosis and applications support
  • Merged reality support with Help Lightning – a virtual engineer at your side (video)
  • Planned maintenance, according to your requirement
  • Customer on-site repair and maintenance service
  • Spare parts availability typically over night or within a few working days worldwide
  • Compliance services for installation qualification, operational qualification / performance verification
  • Site planning and relocation

Bruker Support

On our support website you will find:

Software Updates 

  • System software updates are available online to registered users.

Documentation

  • Product manuals and installation guides are available online to registered users.

Labscape

Service & Life Cycle Support for Magnetic Resonance and Preclinical Imaging

Bruker’s commitment to provide customers with unparalleled help throughout the buying cycle, from initial inquiry to evaluation, installation, and the lifetime of the instrument is now characterized by the LabScape service concept.

LabScape Maintenance Agreements, On-Site On-Demand and Enhance Your Lab are designed to offer a new approach to maintenance and service for the modern laboratory