Proteomic Forum/EuPA 2022

Application of a library-free dia-PASEF approach for high throughput and high sensitivity proteomics

Compared dia-PASEF and prm-PASEF approaches for the absolute quantification of 500 human plasma proteins in colon cancer plasma samples

Evaluation of prm-PASEF on a newly designed ultra-high sensitivity tims-Q-TOF

High throughput single-shot proteomics on the timsTOF Pro 2

TIMS DIA-NN on the PaSER platform for automated dia-PASEF data processing workflows

TIMS Viz for Mobility Offset Mass Aligned interrogation of complex samples

Ultra-sensitive proteome quantification on the timsTOF SCP mass spectrometer

April 4, 16:00-17:00 CEST

dia-PASEF^® combines the data completeness and analysis depth typical of DIA approaches with the speed and sensitivity of PASEF-based analysis. Learn how the latest data processing advances enable the full exploitation of the benefits of this 4D-acquisition procedure and contribute to further improvements in the analytical pipeline performance.

Speakers:

Dr. Vadim Demichev, MSTARS, Group leader, Quantitative Proteomics, Department of Biochemistry, Charité - Universitätsmedizin Berlin, Germany: Ultra-sensitive proteomics with DIA-NN and dia-PASEF^®

Chris Adams, Ph.D., Business Development Director, Global Bioinformatics, Life Science Mass Spectrometry, Bruker Daltonics, Billerica, MA, USA: dia-PASEF^® combined with TIMScore™ and TIMS DIA-NN for proteome depth at scale

April 5, 12:15-13:15 CEST

Implementing a single cell proteomics analysis capability in a lab requires addressing a number of challenges. These include an efficient sample preparation pipeline for single cells, combined with the highest sensitivity, high-throughput, and high robustness LC-MS, and finally an efficient data processing pipeline. Our in-house scientists, our Cellenion collaboration partners, and one of our most well-known users, Dr. Jenny van Eyk (Director, Precision Biomarker Laboratories, Cedars-Sinai Medical Centre, Los Angeles, USA) will illustrate how those challenges can be addressed with the combined Single Cell proteomics solution provided by Bruker and Cellenion.

Speakers:

Christoph Krisp, Ph.D., Application Scientist Proteomics, Bruker Daltonics GmbH & Co. KG, Bremen, Germany and Dr. David Hartlmayr, Scientist, Cellenion, Lyon, France: Label-free single cell proteomics workflow with the CellenONE platform and the timsTOF SCP

Jennifer van Eyk, Ph.D., Director, Precision Biomarker Laboratories, Cedars-Sinai Medical Centre, Los Angeles, USA: Next steps in personalized medicine

For further information, please contact:

Anne Kropp
Bruker Daltonics GmbH & Co. KG
Fahrenheitstrasse 4
28359 Bremen
anne.kropp@bruker.com