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▶ Watch On-Demand | 70 Minutes

BioAFM and NanoIR: New Insights into Nanomechanical and High-Resolution Chemical Analysis

BioAFM and nanoIR techniques in life science and biomedical research

Watch Individual Sessions:

▶ Watch On-Demand | 25 Minutes

Part 1: The Drug/Metal Nanocarrier Connection: Characterization and Imaging Using Nanoscale Infrared Spectroscopy

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Presented by Natalia Piergies, Ph.D., Assistant Professor, Institute of Nuclear Physics, Polish Academy of Sciences, Poland (June 29, 2021)

PRESENTATION HIGHLIGHTS

  • [00:01:57] Study focus; applications and absorption processes of Erlotinib and Gefitinib
  • [00:04:12] Advantages of metal nanoparticles as potential nanocarriers & considerations for their future use in drug delivery
  • [00:08:15] Review of SERS & SEIRA techniques
  • [00:11:04] Comparing FTIR vs. AFM-IR results
  • [00:13:37] Data and analysis
  • [00:22:37] Conclusions

 

▶ Watch On-Demand | 20 Minutes

Part 2: Probing Bio-Interfaces Using Atomic Force Microscopy and Complementary Techniques

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Presented by Aaron Elbourne, Ph.D., Postdoctoral Research Fellow, School of Science, RMIT University, Australia (June 29, 2021)

PRESENTATION HIGHLIGHTS

  • [00:02:57] Imaging ionic liquids and other viscous materials on surfaces
  • [00:04:18] Imaging deep eutectic solvents (DES)
  • [00:06:16] In situ imaging of virus-like particles
  • [00:08:20] Investigating new cryopreservants for tissue preservation with the JPK NanoWizard 4
  • [00:10:06] Developing antimicrobial treatments to combat biofilm-related antibiotic resistance
  • [00:15:40] Developing nanostructured antimicrobial surfaces
  • [00:19:12] Review of other ongoing AFM-based antimicrobial research
▶ Watch On-Demand | 19 Minutes

Q&A with Session Speakers

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(June 29, 2021)

       QUESTIONS FOR AARON ELBOURNE, PH.D.

  • [00:00:10] How were you able to isolate the virus and what AFM mode & cantilevers were used?
  • [00:02:42] Did you check the loading rate of the bonding maturation during the waiting times?
  • [00:03:21] Did you try different sized nanoparticles of silver or deposition methods?
  • [00:05:00] Do you use the same cantilevers and coating to study bacteria as you use for viruses?
  • [00:05:52] What type of cantilevers do you use?
  • [00:06:38] What is ATR-FTIR's lateral resolution?
  • [00:08:12] Would your experiments also work with gold-coated substrates?

       QUESTIONS FOR NATALIA PIERGIES, PH.D.

  • [00:07:22] What resolution are you typically able to achieve with Tapping AFM-IR?
  • [00:08:45] What is the wavelength range of the laser that you use for nanoscle infrared measurements? In principle, what is the potential achievable wavelength range?
  • [00:10:42] To clarify: There are laser sources available that cover the entire mid-infrared region for both traditional contact-mode AFM-IR and Tapping AFM-IR?

       QUESTIONS FOR BOTH SPEAKERS

  • [00:11:19] What are the depth limits of AFM and AFM-IR techniques? How do you navigate these limits in your research?
  • [00:15:18] A note about the achievable depth and capabilities of our new AFM-IR mode -- Surface Sensitive AFM-IR
  • [00:16:04] How do you prepare the samples for your experiments in liquid?

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