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Tuesday, September 14, 2021 | 10AM PDT | 12PM CDT | 1PM EDT | 7PM GMT+1

Causal Coupling Between Neural Activity, Metabolism, and Behavior Across the Drosophila Brain

presented by Kevin Mann, Ph.D., Post-Doctoral Researcher, Clandinin Lab @ Stanford University

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 In this webinar, Kevin Mann, Ph.D. (Post-Doctoral Researcher, Clandinin Lab @ Stanford University) discusses his latest research and findings using two-photon microscopy to investigate the relationship between neural activity and metabolism. Submit the form for instant, full-length access to this webinar and related resources.


Discover novel applications of two-photon microscopy and optogenetics.

  • Hear about our guest presenter's latest research combining two-photon microscopy with local optogenetic perturbation.
  • Learn about four experimental methods for using two-photon imaging and optogenetics to study the dependence of neuronal activity on metabolic flux in intact circuits on the timescale associated with behavior.
  • Gain new insight into the relationship between neural activity and energy metabolism.
  • Hear our guest speaker answer audience questions about his research, including essential considerations for experimental design.

 

 

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Presenter's Abstract

Coordinated activity across networks of neurons is a hallmark of both resting and active behavioral states in many species. These global patterns alter energy metabolism over seconds to hours, which underpins the widespread use of oxygen consumption and glucose uptake as proxies of neural activity. However, whether changes in neural activity are causally related to metabolic flux in intact circuits on the timescales associated with behavior is unclear.

Here, we combine two-photon microscopy of the fly brain with sensors that enable the simultaneous measurement of neural activity and metabolic flux, across both resting and active behavioral states. We demonstrate that:

  1. Neural activity drives changes in metabolic flux, creating a tight coupling between these signals that can be measured across brain networks.
  2. Even transient increases in neural activity result in rapid and persistent increases in cytosolic ATP, which suggests that neuronal metabolism predictively allocates resources to anticipate the energy demands of future activity (using local optogenetic perturbation).
  3. The initiation of even minimal behavioral movements causes large-scale changes in the pattern of neural activity and energy metabolism, which reveals a widespread engagement of the brain.

As the relationship between neural activity and energy metabolism is probably evolutionarily ancient and highly conserved, our studies provide a critical foundation for using metabolic proxies to capture changes in neural activity.

Guest Speaker

Kevin Mann, Ph.D.
Post-Doctoral Researcher, Clandinin Lab @ Stanford University

Dr. Kevin Mann received Ph.D. degree from the University of California, Berkeley under the guidance of Dr. Kristin Scott where he studied fundamental behaviors in Drosophila using genetics, multiphoton microscopy, and electrophysiology. Next, he moved on to postdoctoral training in the laboratory of Dr. Tom Clandinin at Stanford University. Collaboratively he developed a method for whole-brain calcium imaging to detail the intrinsic functional neuronal network in Drosophila.