Tuesday, September 14, 2021 | 10AM PDT | 12PM CDT | 1PM EDT | 7PM GMT+1

Causal Coupling Between Neural Activity, Metabolism, and Behavior Across the Drosophila Brain

presented by Kevin Mann, Ph.D., Post-Doctoral Researcher, Clandinin Lab @ Stanford University

Submit the Form for Instant, Full-Length Access


In this webinar, Kevin Mann, Ph.D. (Post-Doctoral Researcher, Clandinin Lab @ Stanford University) discusses his latest research and findings using two-photon microscopy to investigate the relationship between neural activity and metabolism. Submit the form for instant, full-length access to this webinar and related resources.


Discover novel applications of two-photon microscopy and optogenetics.

  • Hear about our guest presenter's latest research combining two-photon microscopy with local optogenetic perturbation.
  • Learn about four experimental methods for using two-photon imaging and optogenetics to study the dependence of neuronal activity on metabolic flux in intact circuits on the timescale associated with behavior.
  • Gain new insight into the relationship between neural activity and energy metabolism.
  • Hear our guest speaker answer audience questions about his research, including essential considerations for experimental design.
Please enter your first name
Please enter your last name
Please enter your e-mail address
Please enter a valid phone number
Please enter your Company/Institution
What best describes your current interest?
Please add me to your email subscription list so I can receive webinar invitations, product announcements and events near me.
Please accept the Terms and Conditions

             Privacy Notice   Terms of Use

 

 

* Please fill out the mandatory fields.

Note: You will be redirected to view the webinar after form submission.
After a few moments, you should also receive an email at the address provided that contains a link to re-access the webinar viewing window.

This site is protected by reCAPTCHA and the Google Privacy Policy and Terms of Service apply.

Thank you. Your on-demand video is now available.


DNA-PAINT is a localization-based super-resolution method offering molecular resolution (< 5 nm) combined with unlimited multiplexing capabilities. DNA-PAINT reagents from Massive Photonics are a perfect match for the Bruker Vutara VXL microscope. In combination with the Bruker fluidic system, automated, multiplexed cellular imaging in 3D is now accessible out of the box.

Watch the webinar for practical guidance for DNA-PAINT sample preparation, image acquisition, and data analysis, as well as an introduction to:

  • The Vutara VXL super-resolution microscope and integrated fluidics unit;
  • The theoretical background of DNA-PAINT; and
  • The products available from Massive Photonics for DNA-PAINT experiments.

 

If you have any questions about these or any of our other products or services, please contact us. Follow @BrukerFM on Twitter for event, product, and webinar updates.

Presenter's Abstract

Coordinated activity across networks of neurons is a hallmark of both resting and active behavioral states in many species. These global patterns alter energy metabolism over seconds to hours, which underpins the widespread use of oxygen consumption and glucose uptake as proxies of neural activity. However, whether changes in neural activity are causally related to metabolic flux in intact circuits on the timescales associated with behavior is unclear.

Here, we combine two-photon microscopy of the fly brain with sensors that enable the simultaneous measurement of neural activity and metabolic flux, across both resting and active behavioral states. We demonstrate that:

  1. Neural activity drives changes in metabolic flux, creating a tight coupling between these signals that can be measured across brain networks.
  2. Even transient increases in neural activity result in rapid and persistent increases in cytosolic ATP, which suggests that neuronal metabolism predictively allocates resources to anticipate the energy demands of future activity (using local optogenetic perturbation).
  3. The initiation of even minimal behavioral movements causes large-scale changes in the pattern of neural activity and energy metabolism, which reveals a widespread engagement of the brain.

As the relationship between neural activity and energy metabolism is probably evolutionarily ancient and highly conserved, our studies provide a critical foundation for using metabolic proxies to capture changes in neural activity.

Guest Speaker

Kevin Mann, Ph.D.
Post-Doctoral Researcher, Clandinin Lab @ Stanford University

Dr. Kevin Mann received Ph.D. degree from the University of California, Berkeley under the guidance of Dr. Kristin Scott where he studied fundamental behaviors in Drosophila using genetics, multiphoton microscopy, and electrophysiology. Next, he moved on to postdoctoral training in the laboratory of Dr. Tom Clandinin at Stanford University. Collaboratively he developed a method for whole-brain calcium imaging to detail the intrinsic functional neuronal network in Drosophila.