InVi SPIM
The InVi SPIM
It is perfectly suited for a great variety of samples and applications ranging from cell culture to whole embryos (e.g. Drosophila and Zebrafish). For additional customization options, see the InVi SPIM Lattice Pro.
Illumination and Detection
The InVi SPIM can achieve a resolution down to 255 nm in xy, enabling subcellular resolution in living samples free of phototoxic effects.
A Nikon CFI Plan Fluor 10x W 0.3 NA water immersion objective lens projects the light-sheet on the sample. Detection includes a high numerical aperture Nikon CFI Apo 25x W 1.1 NA water immersion objective lens. An additional magnification changer results in 31.3x and 62.5x total magnification for field view and sampling adjustment according to your experimental needs.
InVi SPIM Design
ACQUIFER HIVE - A Secure, User-Friendly Big-Data Management Solution
Cutting-edge biological imaging like light-sheet microscopy, but also super-resolution or two-photon imaging, generates a vast amount of data required to provide researchers with all the relevant information about their samples. However, storage, transfer, and processing of the data remain a challenge.
Live Sample Applications
Browse a selection of applications data from our customers below. Researchers are using InVi SPIM in a variety of ways including studies in embryogenesis and developmental biology, organoids, cell cultures, neurobiology and neurodevelopment, plants, and more.
Tumorigenesis in Mammary Organoids
Characterization and imaging of stochastic tumorigenesis in mammary organoids. Imaged on the InVi SPIM.
A. Alladin, L. Chaible, L. Garcia del Valle, S. Reither Sabine, M. Loeschinger, M. Wachsmuth, J.K. Hériché, C. Tischer, M, Jechlinger. Tracking cells in epithelial acini by light-sheet microscopy reveals proximity effects in breast cancer initiation. eLife 2020;9:e54066 doi: 10.7554/eLife.54066
Fixed Astrocyte Spheroid
Spheroid stained with anti-GFAP (Alexa 488) to label astrocytes and anti-Neurofilament200 (Alexa555) to label neurons. Imaged on the InVi SPIM.
Courtesy of:
Markus Bruell
AG Leist, University of Konstanz
Konstanz, Germany
Mitosis in HeLa Cells
Mitosis in HeLa cells stained for histone 2B-mCherry (magenta), GFP-tubulin (green) and GFP-tubulin (white, deconvolved).
Imaged on the InVi SPIM Lattice Pro.
Visualization: Imaris (Bitplane).
Courtesy of:
Sabine Reither
European Molecualr Biology Laboratory (EMBL)
Heidelberg, Germany
Sample: HeLa cells (Neumann et al., Nature. 2010 Apr 1;464(7289):721-7)
Mouse Pre-implantation Development
Left: Mouse preimplantation embryos expressing H2B-mCherry. Nuclei tracking from one-cell stage to blastocyst.
Right: Mouse oocytes expressing CENPC-EGFP and H2B-mCherry for kinetochore tracking.
Imaged on the InVi SPIM.
Petr Strnad, et al. (2016). Inverted light-sheet microscope for imaging mouse pre-implantation development. Nature Methods 13, 139-145
Arabidopsis Root
Transgenic Arabidopsis root expressing a membrane marker. Imaged with the InVi SPIM.
Courtesy of:
Alexis Maizel
COS, University of Heidelberg
Heidelberg, Germany
C. elegans Skeleton Protein Dynamics
Protein dynamics in C. elegans skeleton. Imaged on the InVi SPIM.
Courtesy of:
Dr. Chai Yongping and Dr. Ou Guangshuo
Tsinghua University
China
Specifications
| Illumination | Detection |
Effective Magnification |
Field of View | Pixel Size | Optical Resolution |
| 10x / 0.3 NA | Nikon 25x / 1.1 NA | 31.3x 62.5x |
420 µm 210 µm |
208 nm 104 nm |
255 nm |
Illumination Optics
- Chromatic correction from 440 to 660 nm
- Light-sheet generation by beam scanning
- Flexible light-sheet thickness (2 µm to 6 µm)
- Nikon CFI Plan Fluor 10x W 0.3 NA water immersion objective lens
Detection Optics
- Nikon CFI Apo 25x W 1.1 NA water immersion objective lens
- 2 spectral detection channels, each equipped with a fast filter wheel (10 positions and 50 ms switching time between adjacent positions)
- Filters adapted to the selected laser lines
- 2 high-speed sCMOS cameras Hamamatsu Orca Flash 4.0 V3
- Maximum frame rate >80 fps at full frame (2048 × 2048 pixels of 6.5 µm × 6.5 µm size) and up to 500 fps at subframe cropping
- Peak quantum efficiency (QE): 82% @ 560 nm
