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Luxendo Light-Sheet Microscopes

InVi SPIM

The inverted view light-sheet microscope
InVi SPIM (Inverted View Light-Sheet Microscope)

Highlights

The InVi SPIM

It is perfectly suited for a great variety of samples and applications ranging from cell culture to whole embryos (e.g. Drosophila and Zebrafish).

Features

Illumination and Detection

This configuration not available in Germany.

The InVi SPIM can achieve a resolution down to 255 nm in xy, enabling subcellular resolution in living samples free of phototoxic effects.

A Nikon CFI Plan Fluor 10x W 0.3 NA water immersion objective lens projects the light-sheet on the sample. Detection includes a high numerical aperture Nikon CFI Apo 25x W 1.1 NA water immersion objective lens. An additional magnification changer results in 31.3x and 62.5x total magnification for field view and sampling adjustment according to your experimental needs.

InVi SPIM Design

Not available in Germany

Lux DATA - A Comprehensive Data Processing and Storage Solution

Cutting-edge biological imaging like light-sheet microscopy, but also super-resolution or two-photon imaging, generates a vast amount of data required to provide researchers with all the relevant information about their samples. However, storage, transfer, and processing of the data remain a challenge.

Applications

Live Sample Applications

Browse a selection of applications data from our customers below. Researchers are using InVi SPIM in a variety of ways including studies in embryogenesis and developmental biology, organoids, cell cultures, neurobiology and neurodevelopment, plants, and more.

Tumorigenesis in Mammary Organoids

Characterization and imaging of stochastic tumorigenesis in mammary organoids. Imaged on the InVi SPIM.  

A. Alladin, L. Chaible, L. Garcia del Valle, S. Reither Sabine, M. Loeschinger, M. Wachsmuth, J.K. Hériché, C. Tischer, M, Jechlinger. Tracking cells in epithelial acini by light-sheet microscopy reveals proximity effects in breast cancer initiation. eLife 2020;9:e54066 doi: 10.7554/eLife.54066

 

Fixed Astrocyte Spheroid

Spheroid stained with anti-GFAP (Alexa 488) to label astrocytes and anti-Neurofilament200 (Alexa555) to label neurons. Imaged on the InVi SPIM.

Courtesy of:
Markus Bruell
AG Leist, University of Konstanz
Konstanz, Germany

Mitosis in HeLa Cells

Mitosis in HeLa cells stained for histone 2B-mCherry (magenta), GFP-tubulin (green) and GFP-tubulin (white, deconvolved).

Imaged on the InVi SPIM Lattice Pro.

Visualization: Imaris (Bitplane).

Courtesy of:
Sabine Reither
European Molecualr Biology Laboratory (EMBL)
Heidelberg, Germany

Sample: HeLa cells (Neumann et al., Nature. 2010 Apr 1;464(7289):721-7)

Mouse Pre-implantation Development

Left: Mouse preimplantation embryos expressing H2B-mCherry. Nuclei tracking from one-cell stage to blastocyst.

Right: Mouse oocytes expressing CENPC-EGFP and H2B-mCherry for kinetochore tracking.

Imaged on the InVi SPIM.

Petr Strnad, et al. (2016). Inverted light-sheet microscope for imaging mouse pre-implantation development. Nature Methods 13, 139-145

 

Arabidopsis Root

Transgenic Arabidopsis root expressing a membrane marker. Imaged with the InVi SPIM.

Courtesy of:
Alexis Maizel
COS, University of Heidelberg
Heidelberg, Germany

C. elegans Skeleton Protein Dynamics

Protein dynamics in C. elegans skeleton. Imaged on the InVi SPIM.

Courtesy of:
Dr. Chai Yongping and Dr. Ou Guangshuo
Tsinghua University
China

Specifications

Specifications

Illumination Detection Effective Magnification Field of View Pixel Size Optical Resolution
10x / 0.3 NA Nikon 25x / 1.1 NA

31.3x

62.5x

420 µm

210 µm

208 nm

104 nm

255 nm

Illumination Optics

  • Chromatic correction from 440 to 660 nm
  • Light-sheet generation by beam scanning
  • Flexible light-sheet thickness (2 µm to 6 µm)
  • Nikon CFI Plan Fluor 10x W 0.3 NA water immersion objective lens

Detection Optics

  • Nikon CFI Apo 25x W 1.1 NA water immersion objective lens
  • 2 spectral detection channels, each equipped with a fast filter wheel (10 positions and 50 ms switching time between adjacent positions)
  • Filters adapted to the selected laser lines
  • 2 high-speed sCMOS cameras Hamamatsu Orca Flash 4.0 V3
  • Maximum frame rate >80 fps at full frame (2048 × 2048 pixels of 6.5 µm × 6.5 µm size) and up to 500 fps at subframe cropping
  • Peak quantum efficiency (QE): 82% @ 560 nm

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