This webinar focuses on the basic requirements and practical considerations necessary to obtain high-quality single-molecule localization microscopy data while imaging fixed cells.
Single-molecule localization microscopy (SMLM) is an optical super-resolution technique that circumvents the diffraction limit of light by localizing the center point of each fluorophore’s recorded point spread function. SMLM requires the use of labeling strategies and specialized fluorophores and/or buffer conditions that enable most of the fluorophores in the sample to be “turned off” during the experiment so that a sparse subset of the fluorophores in the sample can be isolated from their nearest “on” neighbors and localized to build up an image of interest. Due to the nature of super-resolution single-molecule imaging, careful sample preparation, imaging buffer preparation, and optimized imaging conditions are fundamental requirements for obtaining quality data.
In this webinar, we cover the basic requirements for fixed cell imaging that a researcher will need to obtain high-quality single-molecule localization microscopy data.
At the conclusion of the webinar attendees should leave with a better understanding of the factors that need to be considered when preparing samples and acquiring data for SMLM.
Find out more about the technology featured in this webinar or our other fluorescence microscopy solutions:
Dr. Lauren Gagnon
Vutara Applications Scientist, Bruker