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Light-Sheet Images of Organoids and Cell Cultures

3D Imaging of Organoids

Pancreatic Sphere

hESC-derived pancreatic spheres. Imaging on the TruLive3D Imager enables collecting information of several samples in one experiment. Visualization: Imaris (Bitplane)

Courtesy of:
Yung Hae Kim
Graphin-Botton Group, MPI-CBG
Dresden, Germany

 

Tumorigenesis in Mammary Organoids

Characterization and imaging of stochastic tumorigenesis in mammary organoids. Imaged on the InVi SPIM.  

A. Alladin, L. Chaible, L. Garcia del Valle, S. Reither Sabine, M. Loeschinger, M. Wachsmuth, J.K. Hériché, C. Tischer, M, Jechlinger. Tracking cells in epithelial acini by light-sheet microscopy reveals proximity effects in breast cancer initiation. eLife 2020;9:e54066 doi: 10.7554/eLife.54066

 

3D Cell Culture

3D culture system of human primary cells imaged on the QuVi SPIM.

Courtesy of:
Yassen Abbas
Turco Lab, University of Cambridge
Cambridge, UK

 

Fixed Astrocyte Spheroid

Spheroid stained with anti-GFAP (Alexa 488) to label astrocytes and anti-Neurofilament200 (Alexa555) to label neurons. Imaged on the InVi SPIM.

Courtesy of:
Markus Bruell
AG Leist, University of Konstanz
Konstanz, Germany

3D Cell Culture

3D culture system of human primary cells imaged on the QuVi SPIM (330µm x 220 µm x 1200µm). Imaged on the QuVi SPIM.

Courtesy of:
Yassen Abbas
Turco Lab, University of Cambridge
Cambridge, UK

3D Imaging of a Spheroid

Spheroid labeled with EGFP and mRFP imaged on the InVi SPIM Lattice Pro. Three illumination patterns were tested for each label: Gaussian beams, Bessel beams, and optical lattices. The optical lattices gave the best results for the EGFP labeling, while the Gaussian beam was optimal for the mRFP labeling.

Courtesy of:
Martin Stöckl
University of Konstanz
Germany

Colonies of Mouse Embryonic Stem Cells

Colonies of mouse embryonic stem cells stably expressing H2B-mCherry and IRFP670 with a membrane-targeting signal. Imaged on the InVi SPIM.

Courtesy of:
Pierre Neveu
European Molecular Biology Laboratory (EMBL)
Heidelberg, Germany

Cell Culture Imaging

Mitosis in HeLa Cells

Mitosis in HeLa cells stained for histone 2B-mCherry (magenta), GFP-tubulin (green) and GFP-tubulin (white, deconvolved).

Imaged on the InVi SPIM Lattice Pro.

Visualization: Imaris (Bitplane).

Courtesy of:
Sabine Reither
European Molecualr Biology Laboratory (EMBL)
Heidelberg, Germany

Sample: HeLa cells (Neumann et al., Nature. 2010 Apr 1;464(7289):721-7)

Mouse Pre-implantation Development

Left: Mouse preimplantation embryos expressing H2B-mCherry. Nuclei tracking from one-cell stage to blastocyst.

Right: Mouse oocytes expressing CENPC-EGFP and H2B-mCherry for kinetochore tracking.

Imaged on the InVi SPIM.

Petr Strnad, et al. (2016). Inverted light-sheet microscope for imaging mouse pre-implantation development. Nature Methods 13, 139-145

 

Epithelial Cells

Epithelial cell line (BS-C-1) stained for f-actin and chromatin. Image taken with InVi SPIM Lattice Pro.

Courtesy of:
Ulrike Engel
Nikon Imaging Center
University of Heidelberg
Germany

HeLa Cell Culture

HeLa cells expressing GFP and mCherry. Imaged on the InVi SPIM.

Courtesy of:
Tobias A. Knoch
Erasmus MC
Rotterdam, The Netherlands