Light-Sheet Images of Organoids and Cell Cultures

Light-sheet microscopes for investigating 3D cell cultures, such as organoids and spheroids, long-term with environmental control.

Image Structure and Behavior of 3D Cell Cultures

In recent years, 3D cell cultures have gained popularity due to their clear potential to mimic a realistic in vivo tissue in terms of structure, architecture, multi-cellular microenvironments and function. Established 3D cell culture systems such as organoids and spheroids have been proven to be valuable tools in applications including disease modeling, cancer research (e.g. tumor biology and host-tumor interactions), drug development and testing, as well as regenerative medicine.

Light-sheet microscopes are the most powerful tools for investigating the complex structure and behavior of 3D cell cultures by performing live, fast, long-term and high-resolution imaging with minimal phototoxic effects.

Bruker offers different light-sheet geometries to enable multiplexing and gentle, long-term 3D cell culture imaging under precisely controlled environmental conditions.

3D Imaging of Organoids

Pancreatic Sphere

hESC-derived pancreatic spheres. Imaging on the TruLive3D Imager enables collecting information of several samples in one experiment. Visualization: Imaris (Bitplane)

Courtesy of:
Yung Hae Kim
Graphin-Botton Group, MPI-CBG
Dresden, Germany


Tumorigenesis in Mammary Organoids

Characterization and imaging of stochastic tumorigenesis in mammary organoids. Imaged on the InVi SPIM.  

A. Alladin, L. Chaible, L. Garcia del Valle, S. Reither Sabine, M. Loeschinger, M. Wachsmuth, J.K. Hériché, C. Tischer, M, Jechlinger. Tracking cells in epithelial acini by light-sheet microscopy reveals proximity effects in breast cancer initiation. eLife 2020;9:e54066 doi: 10.7554/eLife.54066


3D Cell Culture

3D culture system of human primary cells imaged on the QuVi SPIM.

Courtesy of:
Yassen Abbas
Turco Lab, University of Cambridge
Cambridge, UK


Fixed Astrocyte Spheroid

Spheroid stained with anti-GFAP (Alexa 488) to label astrocytes and anti-Neurofilament200 (Alexa555) to label neurons. Imaged on the InVi SPIM.

Courtesy of:
Markus Bruell
AG Leist, University of Konstanz
Konstanz, Germany

3D Cell Culture

3D culture system of human primary cells imaged on the QuVi SPIM (330µm x 220 µm x 1200µm).

Courtesy of:
Yassen Abbas
Turco Lab, University of Cambridge
Cambridge, UK

3D Imaging of a Spheroid

Spheroid labeled with EGFP and mRFP imaged on the InVi SPIM Lattice Pro. Three illumination patterns were tested for each label: Gaussian beams, Bessel beams, and optical lattices. The optical lattices gave the best results for the EGFP labeling, while the Gaussian beam was optimal for the mRFP labeling.

Courtesy of:
Martin Stöckl
University of Konstanz

Colonies of Mouse Embryonic Stem Cells

Colonies of mouse embryonic stem cells stably expressing H2B-mCherry and IRFP670 with a membrane-targeting signal. Imaged on the InVi SPIM.

Courtesy of:
Pierre Neveu
European Molecular Biology Laboratory (EMBL)
Heidelberg, Germany

Cell Culture Imaging

Mitosis in HeLa Cells

Mitosis in HeLa cells stained for histone 2B-mCherry (magenta), GFP-tubulin (green) and GFP-tubulin (white, deconvolved).

Imaged on the InVi SPIM Lattice Pro.

Visualization: Imaris (Bitplane).

Courtesy of:
Sabine Reither
European Molecualr Biology Laboratory (EMBL)
Heidelberg, Germany

Sample: HeLa cells (Neumann et al., Nature. 2010 Apr 1;464(7289):721-7)

Mouse Pre-implantation Development

Left: Mouse preimplantation embryos expressing H2B-mCherry. Nuclei tracking from one-cell stage to blastocyst.

Right: Mouse oocytes expressing CENPC-EGFP and H2B-mCherry for kinetochore tracking.

Imaged on the InVi SPIM.

Petr Strnad, et al. (2016). Inverted light-sheet microscope for imaging mouse pre-implantation development. Nature Methods 13, 139-145


Epithelial Cells

Epithelial cell line (BS-C-1) stained for f-actin and chromatin. Image taken with InVi SPIM Lattice Pro.

Courtesy of:
Ulrike Engel
Nikon Imaging Center
University of Heidelberg

HeLa Cell Culture

HeLa cells expressing GFP and mCherry. Imaged on the InVi SPIM.

Courtesy of:
Tobias A. Knoch
Erasmus MC
Rotterdam, The Netherlands