The InVi SPIM Lattice Pro provides the highest level of flexibility for illuminating samples using a proprietary Advanced Illumination Module (AIM). It expands the capabilities of the InVi SPIM: while maintaining the ease-of-use and stability of the system, it adds tailorable, interactive adaptability of the beam shape to suit the highly specific requirements of your sample. You choose what gives you the best results for your 3D high resolution imaging experiment – large field of view, high speed or optimal spatial resolution.
The InVi SPIM Lattice Pro offers a variety of illumination patterns, ranging from the classical static Gaussian light-sheet or a scanned Gaussian beam to sophisticated illumination schemes like Bessel beams, Airy beams or optical lattices.
The user can select from a broad choice of beam shapes to improve the microscope’s resolution and reduce photo-damage in delicate samples. A Special Optics 28.6x 0.7 NA water immersion objective lens projects the light-sheet on the sample. A high numerical aperture Nikon CFI Apo 25x W 1.1 NA water immersion objective lens images the signal onto one or two Hamamatsu sCMOS cameras. An additional magnification changer provides 31.3x and 62.5x total magnification to allow you to optimize field of view and pixel size to your experimental needs.
Browse a selection of applications data from our customers below. Researchers are using the InVi SPIM Lattice Pro in a variety of ways including studies in embryogenesis and developmental biology, organoids, cell cultures, neurobiology and neurodevelopment, plants, and more.
Mitosis in HeLa cells stained for histone 2B-mCherry (magenta), GFP-tubulin (green) and GFP-tubulin (white, deconvolved).
Imaged on the InVi SPIM Lattice Pro.
Visualization: Imaris (Bitplane).
Courtesy of:
Sabine Reither
European Molecualr Biology Laboratory (EMBL)
Heidelberg, Germany
Sample: HeLa cells (Neumann et al., Nature. 2010 Apr 1;464(7289):721-7)
Spheroid labeled with EGFP and mRFP imaged on the InVi SPIM Lattice Pro. Three illumination patterns were tested for each label: Gaussian beams, Bessel beams, and optical lattices. The optical lattices gave the best results for the EGFP labeling, while the Gaussian beam was optimal for the mRFP labeling.
Courtesy of:
Martin Stöckl
University of Konstanz
Germany
HeLa cells expressing GFP and mCherry. Imaged on the InVi SPIM.
Courtesy of:
Tobias A. Knoch
Erasmus MC
Rotterdam, The Netherlands
Illumination | Detection |
Effective Magnification |
Field of View | Pixel Size | Optical Resolution |
28.6x / 0.7 NA | Nikon 25x / 1.1 NA | 31.3x 62.5x |
420 µm 210 µm |
208 nm 104 nm |
255 nm |
Illumination Optics
Detection Optics
Bruker's LuxBundle software saves time and enhances productivity by providing:
MuVi SPIM records a sample from different angles/views and generates images composed of multiple tiles. The LuxBundle software ensures high-quality, 360° crisp images of the sample that compensate for absorption and scattering. Features include:
LuxBundle's integrated 3D data viewer allows researchers to inspect the entire dataset directly after acquisition. This gives users control over their data with key capabilities, including: