Multiphoton Microscopy Applications

Voltage Imaging

Simultaneous recording of membrane potential from multiple neurons with an improved signal-to-noise ratio in vivo

A technology that simultaneously records membrane potential from multiple neurons in behaving animals will have a transformative effect on neuroscience research (1,2). Genetically encoded voltage indicators are a promising tool for these purposes; however, these have so far been limited to single-cell recordings with a marginal signal-to-noise ratio in vivo.

Bruker is actively developing solution for our customers pursuing this application. Please contact Bruker for more details or for opportunity to collaborate on this fascinating project.

The Ultima 2Pplus enabled advanced neuroscience research for Victor Hugo Cornejo's lab in their recent publication, "Voltage compartmentalization in dendritic spines in vivo," where they measured membrane potentials in spines and dendrites in the somatosensory cortex of mice during spontaneous activity and sensory stimulation. To investigate these electrical functions in vivo, they developed a genetically encoded voltage indicator (GEVI) and then used two-photon imaging to measure voltage dynamics. Findings indicate that spines can compartmentalize voltage in physiological states in vivo and has important implications for future studies with synaptic function, synaptic plasticity, and dendritic integration during neurological diseases.