Super-Resolution Microscopy Resource Library

Webinar Recap: Single-Molecule Localization Microscopy—Beyond the Coverslip

This webinar recap summarizes a presentation given by Lauren Gagnon, Applications Scientist for Bruker’s super-resolution microscopy solutions, on the use of single-molecule localization for high-resolution biological imaging.

Discover how scientists are leveraging super-resolution microscopy to unveil nanoscale structures in biological samples.

In our "Single-Molecule Localization Microscopy—Beyond the Coverslip" webinar, Lauren Gagnon, Applications Scientist at Bruker, explores the principles and breakthroughs of SMLM, showcasing its ability to image labeled biological structures down to ~20 nm resolution.

Bruker’s Vutara system combines bi-plane imaging and top-hat illumination to deliver uniform 3D imaging across diverse sample types—from individual cells to whole organisms. These technologies open new avenues for high-resolution research in genomics, neurobiology, and developmental biology.

FROM THE WEBINAR

Audience Q&A

Download the webinar recap or watch the recording to learn more about these and other topics.

  1. Do you need to run calibrations regularly?
  2. How much depth can you cover?
  3. What do you use as a spatial filter?
  4. Is it possible to image a live cell?
  5. What is the spatial resolution that you can reach with the system?
  6. Do we need to use specific fluorophores for imaging on a Super-Resolution Vutara System?
  7. Can the Software run on a Mac?
  8. How fast is image acquisition and how does photobleaching affect the acquisition of image quality?

If you missed this event, you can watch the recording here: