Imaging in Developmental & Cell Biology

Life is on in 3D - just reveal it in real time! Live-cell imaging is currently an integral part of modern developmental & cell biology. It enables the precise tracking of highly complex and dynamic molecular and cellular events during embryonic development and in cell culture applications. Light-sheet microscopy is the most powerful method to unveil the complex structure and behavior of whole embryos and cell cultures.  It enables collecting meaningful live-cell imaging microscopy data by providing   fast, long-term and high-resolution imaging while minimizing phototoxic effects and keeping environmental conditions stable. 

Bruker offers different light-sheet geometries for long-term rapid and gentle 3D imaging of living samples. The unique 4-axis concept of the Luxendo MuVi SPIM LS is the ideal tool for in toto imaging of Drosophila, Zebrafish, and many more sample types. The Luxendo InVi SPIM light-sheet microscopes are designed for live-sample imaging applications ranging from 2D & 3D cell cultures to small embryos, under precisely controlled environmental conditions. Expand your capabilities with the Luxendo InVi SPIM Lattice Pro and its flexible choice of illumination patterns – Gaussian beams, Bessel beams, optical lattices, and more.

Enjoy the flexibility and versatility of Luxendo SPIM solutions in terms of photo-ablation, photo-activation, perfusion etc.  

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Cell Tracking in Drosophila Embryo Development

Cell tracking created with Arivis Vision4D 3D visualization and analysis software. Imaged on the MuVi SPIM.

Courtesy of: Celia Smits and Stanislav Y. Shvartsman, Department of Molecular Biology, Princeton University, NJ, USA

Tumorigenesis in Mammary Organoids

Characterization and imaging of stochastic tumorigenesis in mammary organoids. Imaged on the InVi SPIM.

Taken from:
Tracking cells in epithelial acini by light sheet microscopy reveals proximity effects in breast cancer initiation. Ashna Alladin, Lucas Chaible, Lucia Garcia del Valle, Reither Sabine, Monika Loeschinger, Malte Wachsmuth, Jean-Karim Hériché, Christian Tischer, Martin Jechlinger. eLife 2020;9:e54066 doi: 10.7554/eLife.54066

Tracking Mitosis in HeLa Cell Culture

Mitosis in HeLa cells stained for histone 2B-mCherry (magenta), GFP-tubulin (green) and GFP-tubulin (white, deconvolved). Imaged on the InVi SPIM Lattice Pro. Visualization: Imaris (Bitplane).

Courtesy of: Sabine Reither European Molecualr Biology Laboratory (EMBL) Heidelberg, Germany

Sample: HeLa cells (Neumann et al., Nature. 2010 Apr 1;464(7289):721-7)


Luxendo Light-Sheet Microscopy Systems for Developmental & Cell Biology Applications

Do you want to image delicate cell cultures or whole living model organisms as they develop and grow? Start observing them in their native environment in 3D over days!  Our light-sheet technology is the gentlest way to track these dynamic processes over long periods of time! Our choice of light-sheet microscopy systems enable high-resolution, dual sided-illumination imaging of delicate samples in their native, stable, controlled 3D environment.

MuVi SPIM

MuVi SPIM LS

  • Horizontal setup
  • Dual-sided illumination and dual-sided detection
  • Patented 4D translation /rotation stage to accurately position and rotate the sample
  • Upgrade possibilities: compatible with photoactivation, photoablation, etc.
InVi SPIM

InVi SPIM

  • Inverted setup
  • Small sample medium volume
  • Accurate temperature and atmosphere control
  • Easy sample accessibility
  • Upgrade possibilities: compatible with photoactivation, photoablation, etc.
InVi SPIM Lattice Pro

InVi SPIM Lattice Pro

  • Inverted setup
  • Modular extension of the InVi SPIM
  • Flexible illumination patterns - Gaussian beams, Bessel beams or optical lattices
  • You choose what you need - large field of view, high-speed, or best-in-class spatial resolution

 

Contact us to upgrade your lab with Luxendo light-sheet technology!