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TruLive3D Dishes for 3D Imaging

The NEW commercially available, ready-to-use TruLive3D Dishes for the InVi SPIM systems have been designed to further facilitate sample mounting.

Powered by Ibidi, the dishes are easily exchangeable, sterile, customizable, disposable and biocompatible. Grow your samples on the foil or place them into the dish wells. For imaging, simply slide the dish into the adapter attached to the microscope. The dishes seamlessly integrate into the large chamber of the TruLive3D Imager, which can fit up to three of them.

InVi SPIM Sample Holder with TruLive3D Dish
InVi SPIM sample holder with TruLive3D Dish

Device Design

The InVi SPIM’s compact, vibration-free and robust design provides maximal stability during your long-term experiments.

Tailored to fit your lab bench, this class 1 laser system does not require any air table or vibration-compensation mechanism as all moving components are light-weight and balanced. Maximal stability of focus and thermal conditions are also guaranteed. The proprietary piezo-crawler stages ensure longevity and precision for a permanently accurate specimen positioning. Neither the images nor the natural growth behaviour of your sample are affected by the gentle image recording.

InVI SPIM Platform
InVI SPIM Platform

Illumination and Detection

The illumination optics comprise light-sheet generation by scanning a beam after passing a beam-shaper unit for length and diameter adjustment.

The lenses project two aligned light sheets from opposing directions on the sample. The expandable, alignment-free light source allows a range of up to six lasers (405, 445, 488, 515, 532, 561, 594, 642, and 685 nm with 40 mW each before fiber).

The detection optics include two high numerical aperture immersion objective lenses. The two spatial detection arms are equipped with 10 position high-speed filter wheels, and multiple spectral channels can be acquired sequentially. The robust sCMOS Orca Flash 4.0 V3 cameras from Hamamatsu are well-suited for experiments that require high detection efficiency, quantification and speed.

The sample chamber and all objective lenses are mounted on the octagon, the optical core unit that can be easily removed and replaced while maintaining its optical alignment. This makes the MuVi SPIM very flexible and user-friendly, a big advantage especially in multi-user environments.

Compared to other techniques (e.g. confocal laser scanning and spinning disc microscopy), light-sheet microscopy enables the acquisition of a higher number of time points while minimizing photo-damaging effects. A deeper insight into dynamic biological processes and fast movement tracking can be achieved.

Electronics, Microscope Control and Computer

Luxendo's intuitive user interface offers a simple setup and execution of multidimensional experiments, while real-time control is handled by an embedded controller to ensure microsecond-precision timing independent of the PC’s performance fluctuations.  

Precise timing control of all connected devices is a prerequisite for reliable experimental outcomes. Full control of data streaming to storage as well as GPU-supported image processing further complements the overall performance.

 

 

Browser-based interface
Browser-based interface

Specifications

Electronics, Microscope Software & Computer

  • Embedded microscope software with an open communication interface: documented API, TCP/IP-based communication
  • Flexible GUI for interactive microscope control and experiment design
  • Computer with 128 GB RAM, Intel dual 8 core CPU
  • High-speed RAID controller for data streaming, 8 × 4 TB local storage in RAID O
  • GeForce RTX 2080 graphics card
  • 10 Gbit/s on-board Ethernet port
  • 1 UHTV 40 inch display