Opterra Multipoint Confocal

Opterra II Life Sciences Applications

High-Speed Live-Cell Imaging

Opterra II's gentle imaging at full camera rate facilitates high-speed acquisitions over extended periods of time, enabling quantitative measurement of molecular and ionic concentrations using fluorescent reporter molecules.

Photomanipulation

Opterra II's unique optical design allows FRAP, photoconversion, photoablation, and uncaging to occur simultaneously with imaging. The wide variety of scan methods provides a tool for any photomanipulation protocol. Protocols can be synchronized on a frame basis with imaging, or executed on an asynchronous point-and-shoot basis.

Calcium response in Xenopus embryo after photoablation wounding of two cells. Cytoplasm loaded with GCaMP (green) and cell membranes labelled mCherry (orange). Two-color image pairs collected at 10 fps. Courtesy of Bill Bement, University of Wisconsin.

GFP Rho (green), Cdc42 mCherry (red) and actin BFP (blue). Max projections of 20 plane Z-series collected in 3 seconds, Z-series collected every 10 seconds for 30 minutes. Courtesy of Bill Bement, University of Wisconsin.

CLAMP GFP (green) and membrane RFP (red). Two color images collected at 20 fps. CSHL 2015 Xenopus course. 

Photoconversion with 405 nm laser using Mark Points interactive tool, images collected every 5 seconds for 30 minutes. Courtesy of Bill Bement, University of Wisconsin.

High-Speed Volumetic Imaging

Live-cell imaging can also be performed as part of 4D protocols. Opterra II's piezo stage facilitates the acquisition of 3D stacks at camera frame rates, enabling entire multicolor stacks to be acquired in less than a second for monolayer cells, and in a few seconds for small organisms. This enables scientists to document and measure and catalog the behavior of intracellular components.

3D Montages

Opterra II's fast Z acquisition mode flies through Z-stacks, acquiring 3D montage data sets at unprecedented speeds. The Atlas Imaging module in Prairie View makes setting up 3D stage montages a snap.

Opterra_montage.jpg
Montage of 2 mm x 1.5 mm area at 60x, pancreas tumor section.