Ultima Multiphoton Resonant Scanner

Users of multiphoton imaging, especially for neuroscience applications involving the use of calcium indicators to measure neuronal function, are increasingly utilizing resonant scanners in order to increase image acquisition rates.  Resonant scanners allow acquisition of 512x512 images at 30 fps, and even faster rates with the use of regions of interest (ROIs).

The Ultima Resonant Scanner is an optional add-on to the Ultima scan head, and can be configured with a new system, as well as being upgraded in the field.

Resonant Scanner schematic

The unique design of the Ultima light path allows ROIs with the resonant scanner in both X and Y.  Reducing the scan dimension in Y will result in higher frame rates of hundreds of frames per second.  Reducing the scan in X results in a higher signal to noise ratio as sampling frequency is not reduced with reduced X scan.  Resonant scanner ROIs can be panned and scrolled, and scanning can be zoomed up to 16X.

Mouse somatosensory cortex. Courtesy of Chris Deister, Brown University.

When a photoactivation scanner is present, the resonant scanner can be run concurrently with the photoactivation scanner, allowing simultaneous high speed imaging and photoactivation.

For high speed volumetric imaging, the resonant scanner synchronizes with the Bruker piezo to allow continuous bidirectional volumetric imaging.  

Four planes acquired with resonant scanner and piezo, 7 stacks/second, visual cortex. Courtesy of Michael Goard, MIT.