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Ultima In Vitro Specifications

Download the Ultima family brochure here.

SCAN HEAD

Scanning Method Matched pair of 6 mm Cambridge galvanometers with raster and spiral scanning capabilities
Field of View ~600 μm x ~600 μm with 20x objective
Scan Speed at 1x Zoom Raster scan: 1.65 fps at 512 x 512, >12 fps at 64 x 64
Spiral scan: 6 fps at 512 x 512, ~30 fps at 64 x 64
Scan Customization User-definable straight, freehand and circular (infinite) linescan with included software; user-definable pixels per line and lines per scan from 1 - 2048; up to 128x scan zoom; 360° of scan rotation; point scan
Uncaging Option Second set of matched 3 mm Cambridge galvanometers in the same scan head to provide high-precision visible or multiphoton laser sample ablation
High-Speed Imaging Option 8kHz resonant galvanometer; 30 fps at 512 x 512, >1300 fps at 512 x 8 region of interest
Confocal Option Point scan: Automated pinhole confocal attachment with up to three PMTs
Multipoint scan: Camera-based for high-speed imaging

DETECTORS

Reflected Non-Descanned 1 - 4 hand-picked Hamamatsu Multi-Alkali PMTs; upgradeable to high-sensitivity Hamamatsu GaAsP PMTs
Transmitted Non-Descanned 1 - 2 hand-picked Hamamatsu Multi-Alkali PMTs; upgradeable to high-sensitivity Hamamatsu GaAsP PMTs
Dodt Single Hamamatsu PMT for DIC-like image collection
Transmitted Single Hamamatsu PMT for transmitted light image collection
Confocal Point scan: 1 - 3 hand-picked Hamamatsu Multi-Alkali PMTs; upgradeable to high-sensitivity Hamamatsu GaAsP PMTs
Multipoint: EMCCD
Camera Standard C2 camera port built into scan head for standard OLM image collection

OPTICAL INPUTS

Multiphoton Laser Ultima light path is optimized for multiphoton laser input from 690 - 1300 nm
Epifluorescence Standard epifluorescence head included for fluorescence imaging
Visible Laser Fibered laser inputs for visible laser introduction
Ultima Laser Rating Class 1 (Contains Class 3b & Class 4 lasers)
Helios Laser Rating Class 3b
LED Full-field photoactivation with LED module

PLATFORM AND AUTOMATION

Microscope Nikon Ti-E inverted and Nikon Ni-E upright;
Olympus BX51WI and BX61WI;
Zeiss Axio Observer and Axio Examiner
Specimen Stage Bruker Slim Stage for upright;
ASI stage with piezo insert for inverted
Z-focus Internal Focus Motor for Ti-E, BX 61 and Zeiss;
Bruker z-focus motor for BX51WI and Ni-E;
Bruker z-piezo available on BX51WI, BX61WI and Ni-E

SOFTWARE

Prairie View Imaging Software fully integrated with scan head for easy imaging; customizable scan settings for optimization of specimen excitation; integrated control of laser power and PMT high voltages
Z-Series Easy creation of depth stacks with user customizable slice number, step size and laser power
T-Series Easy creation of complex series involving Z-Series and triggered images
Stage Montage Atlas Imaging simplifies setup and optimizes acquisition of 2D and 3D stage montages
Peripherals Integration Wavelength and power control available for multiphoton and visible laser launches
Photoactivation User-defined points and regions for complex laser blanking
Regions of Interest User-defined regions for faster scanning capabilities
Brightness Over Time Capable of collecting BOT information for user-defined regions over time and/or depth
Voltage Inputs/Outputs Signal inputs and outputs for electrophysiological experiments, stimulus control, and synchronization with internal devices
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