Chromosomal Structure
Mitochondria
DNA-PAINT

Vutara Applications

Localization microscopy brings reductionism to a new level in biology – the ability to image and measure the structural and functional interactions between different molecules in a biological specimen.  The power of localization microscopy can be brought to bear on virtually any cellular model.  

Download Article: Single-Molecule Localization Introduction and Applications


Featured Studies

Chromosome Structure

Visualizing in situ chromosome 3-D structure

Biological System:  Maternal and paternal homologs of in chromosome 19 of PGP1 cells
Color Scale: 14 step serial labelling using Alexa 647 oligonucleotides

 

Read about groundbreaking research involving in situ imaging of chromosome structure

SML in Genomics

 

 

Chromosome Structure

Virology

dSTORM images reveal spatial relationships between viral gRNA and viral proteins

Biological System: hRSV
Green: MTRIPS Cy3B antisense Probe
Red: Alexa 647 F-protein

Alonas, E. et al., "Combining Single RNA Sensitive Probes with Subdiffraction-Limited and Live-Cell Imaging Enables the Characterization of Virus Dynamics in Cells." ACS Nano 8, no. 1 (2014): 302-15. doi:10.1021/nn405998v

SML in Virology

 

 

Spatial relationships between viral gRNA and viral proteins

DNA-PAINT

DNA-PAINT provides increased image resolution, the possibility to multiplex >4 targets.

Biological System: BSC1 cells

Probes: Primary and secondary antibody labeled cells.
Probe 1 (magenta) – primary, mouse anti-clathrin; secondary, anti-mouse docking strand 1; Imaging strand 1 Atto647N.
Probe 2 (Cyan) – primary, rabbit anti tubulin; secondary, anti rabbit docking strand 2; Imaging strand 2 Cy3b

All DNA-PAINT secondary antibodies and imaging DNA strands were purchased from Massive-Photonics.

SML in DNA-PAINT

 

 

 

DNA PAINT BSC1 cells

Developmental Biology - Thick Samples

The Vutara 352 is the only commercially available single molecule localization microscope capable of imaging whole tissue sections or model organisms.

Biological System: Whole mount Drosophila larva

Green: Alexa 488 Frizzled Receptor

Red: DyLight 649 Lamin C

Image courtesy of Dr. Sean Speese, OHSU

 

 

 

Whole mount Drosophila larva

Neuroscience – Caenorhabditis elegans

Synaptic labelling in the model organism C. elegans to study the relationship of synaptic calcium channels to the synaptic active zone.

Biological System: Caenorhabditis elegans

Yellow: Skylan-s Active zone marker

Magenta: HaloTag::JF646 Calcium Channel 1

Cyan: SNAPf::JF549 Calcium Channel 2

Image courtesy of Sean Merrill and Dr. Erik Jorgensen University of Utah

Caenorhabditis elegans Neuroscience

Oncology

Routine three color experiment with the Vutara 352

Biological System: U2OS cells

Yellow: ATTO 488 tubulin

Cyan: Cy3B Midbody protein

Magenta: AF647 Midbody protein

Images courtesy of Leremy Colf and Dr. Wes Sundquist University of Utah

 

 

Cy3B Midbody protein

Live Cell

Hi speed sensitive cameras and expert experimental advice from the Vutara team allow for long term stable live cell single molecule localization microscopy.

Biological system: U2OS cells

Label: tom20::HaloTag::GBJF549

Sample is imaged live continuously for 30 min. Each frame represents 100 frames of single molecule localization data binned together to create the frames (2 seconds/frame).

Dyes and cells courtesy of Dr. Luke Lavis and Dr. Fadi Jradi HHMI

SML Live Cell Imaging

Live cell