MALDI Biotyper Systems RUO


Selective testing of antibiotic resistance using MALDI-TOF mass spectrometry

To aid the fight against resistant bacteria, Bruker has developed the MBT STAR-BL software module. This software expands the capabilities of the MALDI Biotyper beyond the identification of microorganisms by enabling fast and accurate analysis of the level of β-lactamase activity in bacterial cultures in microbiological research.

To analyze β-lactamase activity, bacteria samples are co-incubated with a β-lactam antibiotic. Bacteria with β-lactamase activity will be able to hydrolyze the antibiotic. This hydrolyzation is coupled with a mass shift of the molecule which can be measured using mass spectrometry.

Selective testing of antibiotic resistance using MALDI-TOF mass spectrometry
Hydrolysis of the ß-lactam ring of ß-lactam antibiotics (click to enlarge)

After incubation, cell-free supernatant is spotted onto a MALDI target plate and spectra of the antibiotic are acquired. After spectra acquisition, the MBT STAR-BL software module automatically calculates the summed hydrolyzed and non-hydrolyzed (intact) antibiotic signal intensities and the corresponding ratio. Results are displayed as a plot that enables at-a-glance evaluation. 

The assays can conveniently be performed within approximately one hour by utilizing the easy to use MBT STAR-Carba and MBT STAR-Cepha Kits, for rapid detection of carbapenemase and cephalosporinase activity respectively, starting from a culture plate or Sepsityper pellet.

To download the brochure click here.

(click to enlarge)

Not for use in clinical diagnostic procedures.