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Fast Tapping on BioScope Resolve

High-Speed Imaging Without Compromise!

  • Highest resolution imaging of biomolecules on timescales relevant to dynamic biological processes (1 fps scan rates)
     
  • Fastest scanning of any BioAFM while retaining the large XYZ-range required for imaging mammalian cells
     
  • Simplest microscope setup — No additional hardware required for high-speed imaging of single molecules and live-cell studies
     
  • Most complete integration with light microscopy for direct correlation between time-lapse AFM images and optical data
     
  • Available on all BioScope Resolve AFM systems

 

Contact an Expert 

BioScope Resolve Fast Tapping

Dr. Alice Pyne on BioScope

 

Complete Solution for High-resolution, High-speed Studies of Dynamic Biological Processes

Molecular Resolution Imaging at Fast Scan Rates of 1 fps

Label-free AFM studies enable imaging of single biomolecule dynamics at resolutions that can often be challenging for traditional optical microscopy techniques. The high-resolution capabilities of Fast Tapping provide opportunities for studying molecular binding events in real-time under near-physiological conditions. Fast Tapping can be used to study the dynamics of triplex DNA. The location of the triplex region is clearly and stably resolved at high scan rates of 1 fps, even though it is only several nanometers in length and just a few Angstroms in height. Using fast scanning to understand the mechanisms of how these structures are formed can provide insights into their potential use in gene therapy.

 

 

BioScope Resolve Fast Tapping

Left: Fast Tapping movie of a DNA mini-circle containing a triplex region (*) captured in fluid. Image size: 80nm. Right: Simulation showing the interaction between a 15bp triplex-forming oligonucleotide (green) and a DNA mini-circle.

AFM data courtesy of Dr. Alice Pyne, Research Fellow, UKRI/MRC; Simulation courtesy of Dr. Agnes Noy, Research Fellow, Univ. of York

FastTapping DNA Quadraplex OpenClosed Figure
BioScope Resolve Fast Tapping

Fast Tapping movies of a DNA mini-circle with open ssDNA region (left) and another mini-circle in which the quadruplex region undergoes conformational changes (right). Image size 50nm.

Data courtesy of Dr. Alice Pyne, Research Fellow, UKRI/MRC

Ultimate Force Control for Imaging of Dynamic Processes

In AFM studies of biomolecular self-assembly, individual molecules must be loosely bound to a substrate (eg. mica) to allow them to undergo the conformational changes required to form proper intermolecular bonds. However, this makes AFM imaging challenging, as these highly mobile molecules can easily be distorted or dislodged from the substrate surface.

Such is the case in DNA quadruplex formation. The highly sensitive force control of Fast Tapping allows one to follow the folding and unfolding dynamics of the quadruplex region at imaging speeds of 1 fps without distorting or dislodging the DNA. The ability to study the formation and resulting structures of these DNA quadruplexes can help to better understand their related biological functions.

 

 

 

Artifact-free Imaging of Complex Biomolecular Structures

Biological assemblies cover a wide-range of sizes and structural complexities. This often results in large height variations across a sample surface (eg. cells and tissues) that can be difficult to image with standard AFM systems at typical scan speeds. With a large Z-range and optimized feedback loop, Fast Tapping on BioScope Resolve enables routine high-resolution imaging of the most demanding bio-structures even at high-speed scan rates.

 

 

 

FastTapping Collagen Air Figure 2
BioScope Resolve Fast Tapping

Fast Tapping movie of collagen fibers captured at 98Hz (2.5 sec/frame) as compared to images captured at 2Hz (~2min/frame). Image size: 1µm.

 

BioScope Resolve Fast Tapping

Hi-Res Fast Tapping movies of live cells obtained at 12.5 sec/frame (left: 37µm scan) and 3.6 sec/frame (right: 7µm scan) showing membrane ruffling and dynamic actin cytoskeleton rearrangement.

Sample courtesy of Dr. Ivan Liashkovich and Dr. Hermann Schillers, University of Münster, Germany

The Only BioAFM with Full XYZ Range for Cell Dynamics

BioScope Resolve is the only BioAFM that enables fast scanning while simultaneously providing the large piezo scanner range necessary for studying the dynamic behavior of living cells on relevant timescales. The Fast Tapping capabilities of Resolve provide the unique ability to conduct high-resolution studies of the dynamics events involved in various cellular processes such as migration, which includes membrane ruffling and associated rearrangement of the actin cytoskeleton. Together with the most advanced integration of optical microscopy techniques and environmental control, live cell studies have never been easier.

 

 


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