During this webinar, attendees learned about the advantages and applications of using light-sheet microscopy for imaging cleared samples. Key topics discussed include:
Light-sheet fluorescence microscopy serves to image a great variety of large biological samples. It works by having a sheet of laser light illuminate only a thin slice of a fluorescently labeled sample. Decoupling the illumination from the detection, typically via an orthogonal arrangement, enables fast, true volume, in-depth imaging with very low photodamage and bleaching effects.
Clearing techniques have become a valuable tool for applications in 3D microstructure analysis of tissues. They modify the optical properties of usually opaque samples to render them transparent while keeping their structure and fluorescent labels intact. Clearing techniques can be combined with light-sheet microscopy to enable fast, long-term, confocal-like optical sectioning, and high-quality 3D imaging of cleared samples.
Find out more about the technology featured in this webinar or our other solutions for Cleared Sample Imaging:
Dr. Dane Maxfield
Sales and Applications Specialist at Bruker Nano Surfaces