▶ Watch On-Demand | 40 Minutes

On-Demand Session: Causal Coupling Between Neural Activity, Metabolism, and Behavior Across the Drosophila Brain

Find out what multiphoton microscopy can reveal about neural activity and metabolism. 
▶ Watch On-Demand | 28 Minutes

Part 1: Technical Presentation

presented by Kevin Mann, Ph.D., Post-Doctoral Researcher, Clandinin Lab @ Stanford University (September 14, 2021)

       PRESENTATION HIGHLIGHTS:

  • [00:01:08] Introduction to the neural activity/metabolism relationship
  • [00:03:04] Experimental methods for optical investigation of this relationship
  • [00:05:54] Using whole-brain imaging methods to investigate functional neural and metabolic networks
  • [00:11:33] Comparing signals within each brain region to determine the correspondence between neural activity and metabolic flux
  • [00:15:24] Testing the necessity of neural activity for metabolic network structure and sufficiency for neural activity to drive metabolic flux
  • [00:20:03] Using motor behavior to measure the coordination of neural and metabolic activity during action
  • [00:24:02] Other applications
  • [00:25:17] Implications for fMRI research
▶ Watch On-Demand | 12 Minutes

Part 2: Post-Session Q&A with the Speaker

presented by Kevin Mann, Ph.D., Post-Doctoral Researcher, Clandinin Lab @ Stanford University (September 14, 2021)

       PRESENTATION HIGHLIGHTS:

  • [00:00:23] Is there anything that you would like to discuss concerning the sensor you are using, given that the results may be influenced by the specifics of those proteins?
  • [00:02:36] What about your choice of proteins in the optogenetics experiments, specifically?
  • [00:04:02] How does a cell know how much ATP it will need for a specific process or activity?
  • [00:07:06] Are these sensors only in neurons?
  • [00:10:12] Are you going to try Lactate?