xView Module
Enabling Imaging Across Millimeter-Scale Brain Regions
Bruker’s xView™ Module for Ultima 2Pplus multiphoton microscopes delivers a breakthrough in large-scale, high-resolution imaging: this exchangeable lens set extends the field of view (FOV) by more than 2.5x the standard area, enabling researchers to visualize neural activity without sacrificing speed or sensitivity.
This optional add-on module was engineered for advanced systems neuroscience applications and lets researchers study brain-wide connectivity, circuit mapping, and disease mechanisms. It is also uniquely compatible with Bruker’s NeuraLight 3D Ultra® Module for holographic optogenetics, enabling new investigations in functional mapping and manipulation of neuronal networks.
Balance of Resolution and FOV for High-Quality Imaging
Visualize more structures per scan
The xView’s user-exchangeable lens sets allow for choosing the appropriate balance of resolution and FOV for any given experiment. With xView, researchers can achieve the wide FOV typical of a 10X objective while retaining the high numerical aperture (NA) — and the resolution — of a 16X objective.
This field-upgradeable module on Bruker’s Ultima 2Pplus therefore enables broader view studies of dynamics to map cell signaling across regions previously out of view. Furthermore, large tissue scans that may have previously required multiple images are now accessible at higher throughput. Powered by the Ultima xCore FPGA electronics, the xView module enables up to kHz frame rates for regional acquisitions or up to 8K high resolution.
Perform aberration-free imaging with ETL compensation
Patented ETL compensation ensures that large FOV scans with xView have minimal aberrations. Specifically, xView employs Bruker's ETL module to compensate for resolution degradation, which is tightly synchronized and controlled by the Prairie View software. Taken together, xView’s unique capabilities reset the tradeoff limitations between resolution, FOV, and speed.
Explore Emerging Applications
Customizable and versatile technology that grows with your research
The xView module’s unique features enable researchers to explore the ever-expanding scope of applications in neuroscience, cancer biology, and immunology. It provides high-resolution imaging with up to 8K × 8K pixels in galvo mode and 5K × 5K in resonant mode with xCore FPGA electronics. Its exchangeable lens set contains options for FN 28, 36, and 45 mm equivalents so that researchers can pick the best lens for their specific application.
Applications include:
- Mesoscale neural circuit mapping with high spatial and temporal resolution
- Simultaneous large-area imaging and 3D optogenetics for input-output studies
- Investigation of neural disease mechanisms (e.g., epilepsy, Alzheimer’s, and Parkinson’s)
- Large organoid and 3D cell culture imaging
- Whole-tissue montages at higher throughput (e.g., with Atlas Imaging software)
Frequently Asked Questions
Usually, to achieve a large field of view, you have to build a dedicated microscope. Now, with the xView module, the Ultima 2Pplus’s already large field of view has been made even bigger. Uniquely, this doesn’t require a brand-new microscope; it is an add-on that can grow with your research. You can buy the 2Pplus first and later add this module to expand the field of view while maintaining the resolution, sensitivity, and overall image quality that Bruker’s Ultima systems are known for.
The xView includes xView 28, which gives you the same field of view as your current 2Pplus, and if you want larger fields of view, you can pop in the xView 36 or xView 45 lens modules. These are cartridges you can swap in and out to modulate your field of view and resolution. All three lens modules are included.
The xView module is compatible with all standard objective lenses.
Yes, the xView module is only compatible with Bruker Ultima 2Pplus microscopes. It is both field-upgradeable for existing systems and can be added to your purchase of a new Ultima 2Pplus system.
The xView module is exclusively available as an add-on for Ultima 2Pplus multiphoton microscopes. xView can be added to your existing Ultima 2Pplus system or purchased as an add-on with a new Ultima 2Pplus system. It can also be combined with other Bruker multiphoton microscope modules to enable additional capabilities, including optogenetics. When combined with others, it brings even more field of view capability to specific applications. Contact us to discuss your specific application and get expert configuration recommendations.
