Quality Assessment of Biologics: Higher Order Structure (HOS) Analysis Using NMR

The webinar will describe how 2D NMR can offer protein biochemists unprecedented analytical precision. By assigning just one spectrum, users can obtain a unique fingerprint of a protein at an atomistic resolution. This non-destructive technique can also be combined with other complimentary techniques to gain even further insights into sample quality.

Examples of the spectral processing and statistical and chemometric analysis methods being developed to increase automation will also be described. Aside from enabling simultaneous analysis of large data volumes, the automation available today has almost advanced to the point that even non-experts will be able to acquire and analyse NMR spectra for decision making.

Key Topics

You will also learn:

  • 2D NMR spectral fingerprinting for higher order structure analysis of biologics
  • Application to biologics in their formulated state for structural assessment
  • Benefits of the advances in automated data acquisition and analysis
  • Reproducibility and robustness for inter-lab precision
  • Practical, easy application of 2D NMR in the biopharma spa
  • Bruker’s latest instrumentation and software for 2D NMR analysis


John P. Marino

Leader, Biomolecular Structure & Function Group – NIST

Associate Director, Institute for Bioscience and Biotechnology Research - National Institute of Standards and Technology & University of Maryland Since 2008, Dr. Marino has been the leader of the NIST Biomolecular Structure & Function Group and Associate Director of the Institute for Bioscience and Biotechnology Research (IBBR), a joint research institute of the University of Maryland and NIST, in Rockville, Maryland.

Jason S. Wood

Ph.D. Americas Market Manager – Pharma/Biotech

With a Ph.D. in Bio-organic Chemistry (University of Buffalo) and a post-doctoral fellowship at the National Institutes of Health (NIA), Dr. Wood is currently the Regional Market Manager (Americas) at Bruker BioSpin where he manages marketing in the pharma and biotech space.