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rapifleXTM MALDI TissuetyperTM

Speed | Robustness | Definition

速度 | 稳定 | 高清

rapifleX重新定义了MALDI成像的关键性能

  • 每秒钟采集50个真实像素点,能更快得到更好的成像结果
  • smartbeamTM 3D激光,更好的像素与像素间的重复性
  • 采样点间距小于10μm,为目前最高空间分辨率,能得到更真实的生物信息
  • 全新的离子源设计,稳定性更好
rapifleX MALDI Tissuetyper closeup
Smartbeam laser movement

rapifleX MALDI Tissuetyper - Designed for MALDI Imaging

• MALDI-TOF 系统
• 新的离子源得到更好的日间稳定性
• 全新 smartbeam™ 3D 激光
• 高达 50 像素/每秒的采集速度
• 高达 10 kHz 的激光重复频率
• < 10 μm 的像素

Proteins in gastric cancer
Proteins in gastric cancer, pixel size 30 μm, 99000 pixels, 500 laser shots per pixel, measurement time 82 minutes.

专为MALDI组织分型设计的 - smartbeam 3D激光

在样品图像采集时样品台连续移动从而达到高的像素采集速度。新开发的smartbeam 3D激光重复频率高达10 kHz。

新的smartbeam 3D激光束直径可低至5μm,如右图所示,激光可以独立于样品台移动,扫描每个像素的全部区域,得到真实的正方形像素,这能充分检测到所有有效的样品区域,从而得到最高的灵敏度和像素与像素间的重复性。

 

 

Ablated matrix showing square pixels
Ablated matrix showing square pixels.

Integrated software solutions

  • flexImaging 允许建立和分析独立的数据集,可按方法向导方便建立新的实验,全分辨率的图像和加强的虚拟载玻片,能得到详细的组织学注释。
  • SCiLS Lab 用于挖掘 MALDI Tissuetyper 数据的数据统计分析软件,可用于分析单个组织成像数据集,也可用于临床和临床前研究中复杂数据的分析。
Overlay of MALDI Tissuetyper image with microscopic image acquired from the same tissue section provides a clear link to histology
Overlay of MALDI Tissuetyper image with microscopic image acquired from the same tissue section provides a clear link to histology.

新设计的离子源使日间稳定性更好

  • 降低离子源清洗时间,使运行时间得到数量级提升。
    • 当离子源确实需要清洗时,能自动对焦的离子透镜可方便完成拆卸。整个离子透镜可以直接用甲醇淋洗。

rapifleX Tissuetyper
Workflow Ion Source

SCiLS Lab

用于挖掘 MALDI Tissuetyper 数据的数据统计分析软件,可用于分析单个组织成像数据集,也可用于临床和临床前研究中复杂数据的分析。

 

    •  同一个用户界面能同时处理多个数据集
    • 单变量分析功能:平均强度,标准差,发现有差异的生物标志物,假设检验,发现共位置的 m/z 值
    • 无监督多变量分析:大的数据集空间分割,成份分析找出潜在趋势
    • 有监督组织分型:基于训练数据的分类器计算和新的观察数据和样品的分类

Analysis of a clinical dataset in SCiLS Lab
Analysis of a clinical dataset with 21 independent patient samples in SCiLS Lab.

技术领先的用于 MALDI Tissuetyper 的软件

flexImaging

允许建立和分析独立的数据集。

 

    • 可按方法向导方便建立新的实验
    • 64-位软件可以高效的处理百万级像素数据集
    • 可以直接获取单张质谱图
    • 批量采集数据
    • 全分辨率的图像共配准功能和加强的虚拟载玻片,能得到详细的组织学注释

MALDI Imaging data overlaid with virtual microscopic slide in flexImaging
MALDI Imaging data overlaid with virtual microscopic slide in flexImaging.

SCiLS Lab 3D

利用 rapifleX 无与伦比的采集速度,3D MALDI Tissuetyper研究触手可及,SCiLS Lab 3D 可用于组织成像数据重建,得到可视化 3D 图像,并能对数据集进行统计分析。

3D volume reconstruction and spatial segmentation of a kidney sample in SCiLS Lab
3D volume reconstruction and spatial segmentation of a kidney sample in SCiLS Lab.

组织分型 - 一个临床发现的工作流程

rapifleX MALDI Tissuetyper workflow

“The ideal imaging MS system needs high data acquisition speeds, high spatial resolution capabilities, and high mass resolving power. With this new system, Bruker re-imagined the MALDI source and laser optics to deliver very impressive performance and information throughput. This is a revolutionary instrument which has the potential to significantly improve sample throughput and offer useful information in real time in clinical research.”

Dr. Pierre Chaurand, Associate Professor, Université de Montréal, Canada

 

“Translational clinically oriented research with imaging mass spectrometry is critically dependent on speed and robust operation. Current instrumentation is limiting its full adaptation. The rapifleX MALDI Tissuetyper allows us to finally overcome that limitation. It enables us to engage in high throughput clinical studies and establishes mass spectrometry imaging as a powerful and reliable information source for personalized medicine research.“

Dr. Ron M.A. Heeren, Director M4I, Maastricht University, The Netherlands

 

“The analysis of tumor heterogeneity is one of the most important biomedical research challenges in oncology and personalized medicine. MALDI imaging is perfectly suited to get an unbiased molecular view on tumor heterogeneity. The speed and performance of the rapifleX MALDI Tissuetyper gives us for the first time the possibility to analyze even large tumor specimen over huge patient cohorts with sufficient spatial resolution to get a comprehensive understanding of tumor heterogeneity. “

Dr. Axel Walch, Institute of Pathology, Helmholtz-Centre Munich, Germany

服务与支持

  • 服务台帮助您迅速与我们技术支持团队取得联系、快速跟进您的服务订单和查询一般信息
  • 根据您的要求,制定维修计划
  • 来自专业人士的电话支持,能够为您区分并解决软硬件问题
  • 基于 Web 的仪器远程服务,提供维修诊断和应用技术支持
  • 现场维修和维护服务
  • 全球范围内通常仅需隔日或几个工作日,维修配件即可送达
  • 安装确认(IQ)和运行确认/性能验证(OQ/ PV)合规服务
  • 实验场地规划、仪器的搬家及重新安装
  • 提高实验室效率的建议(耗品订购、方法开发以及如何运行布鲁克提供的完整解决方案)

Bruker rapifleX MALDI Tissuetyper - Product Animation

Bruker rapifleX MALDI Tissuetyper pre-introduction day in Bremen

For Research Use Only. Not for Use in Clinical Diagnostic Procedures.