Automated Multiplexed 3D Super-Resolution Cell Imaging with DNA-PAINT
Using DNA-PAINT for impressive super-resolution imaging
Gain firsthand insights from imaging experts into how the localization-based super-resolution microscopy method DNA-PAINT works and how it can contribute to your lab. Topics discussed will include:
- The technical background of how DNA-PAINT works
- How to prepare your samples for DNA-PAINT imaging
- Best practices for image acquisition and target multiplexing
Webinar Summary
Super-resolution imaging techniques have transformed biological and biomedical research by allowing researchers to observe structures well below the classical diffraction limit of light. DNA Points Accumulation for Imaging in Nanoscale Topography (DNA-PAINT) is an easy-to-implement approach to localization-based super-resolution microscopy by using small DNA probes. In DNA-PAINT, transient binding of short dye-labeled oligonucleotides (imager) to their complementary target (docking) strands creates the necessary blinking to enable stochastic super-resolution microscopy. Using the programmability and specificity of DNA molecules as imaging and labeling probes allows researchers to decouple blinking from dye photophysics. This alleviates the limitations of current super-resolution techniques and makes it compatible with virtually any single-molecule compatible dye. Furthermore, because different docking strands can be added to different targets within the same sample, there is unlimited multiplexing potential. In this webinar, we will show you how to perform multiplexed three-dimensional DNA-PAINT measurements on the Vutara VXL with an integrated fluidics unit.
Featured Products & Technology
Speakers
Dr. Jürgen Schmied, CEO and Co-Founder, Photonics
Sebastian Strauss, CTO and Co-Founder, Photonics
Dr. Florian Selbach, CSO, Photonics
Dr. Lauren Gagnon
Vutara Applications Scientist, Bruker
Dr. Lauren Gagnon graduated from Emmanuel College with a degree in Chemistry before completing her PhD in Chemistry at the University of Washington. Her thesis research focused on the development of novel approaches to single molecule localization microscopy using DNA barcoded antibodies.