Automated Multiplexed 3D Super-Resolution Cell Imaging with DNA-PAINT
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DNA-PAINT is a localization-based super-resolution method offering molecular resolution (< 5 nm) combined with unlimited multiplexing capabilities. DNA-PAINT reagents from Massive Photonics are a perfect match for the Bruker Vutara VXL microscope. In combination with the Bruker fluidic system, automated, multiplexed cellular imaging in 3D is now accessible out of the box.
Watch the webinar for practical guidance for DNA-PAINT sample preparation, image acquisition, and data analysis, as well as an introduction to:
- The Vutara VXL super-resolution microscope and integrated fluidics unit;
- The theoretical background of DNA-PAINT; and
- The products available from Massive Photonics for DNA-PAINT experiments.
If you have any questions about these or any of our other products or services, please contact us. Follow @BrukerFM on Twitter for event, product, and webinar updates.
Presenters' Abstract
Super-resolution imaging techniques have transformed biological and biomedical research by allowing researchers to observe structures well below the classical diffraction limit of light. DNA Points Accumulation for Imaging in Nanoscale Topography (DNA-PAINT) is an easy-to-implement approach to localization-based super-resolution microscopy by using small DNA probes. In DNA-PAINT, transient binding of short dye-labeled oligonucleotides (‘imager’) to their complementary target (‘docking’) strands creates the necessary ‘blinking’ to enable stochastic super-resolution microscopy. Using the programmability and specificity of DNA molecules as imaging and labeling probes allows researchers to decouple blinking from dye photophysics, alleviating limitations of current super-resolution techniques, and making it compatible with virtually any single-molecule compatible dye. Further, because different docking strands can be added to different targets within the same sample there is potentially unlimited multiplexing potential.
In this webinar we will show you how to perform multiplexed three-dimensional DNA-PAINT measurements on the Vutara VXL with integrated fluidics unit. We will cover the theoretical background, sample preparation, image acquisition and target multiplexing.
Speakers
Dr. Jürgen Schmied, CEO and Co-Founder, schmied@massive-photonics.com
Sebastian Strauss, CTO and Co-Founder, strauss@massive-photonics.com
Dr. Florian Selbach, CSO, selbach@massive-photonics.com
Laurin Gagnon, Applications Scientist, lauren.gagnon@bruker.com
