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Photothermal AFM-IR Spectroscopy and Imaging for Life Science

with guest speaker Dr. Ariane Deniset-Besseau, Université Paris-Saclay

This webinar will cover numerous applications in the field of life sciences. In order to illustrate the broad applicability, we will discuss selected examples in detail, ranging from tissue to single protein fibrils. Photothermal AFM-IR can provide nanoscale chemical information with highly resolved IR spectra, that directly correlate to FT-IR transmission spectroscopy. The capabilities of the technique will be illustrated through the study of different samples: i) lipids bodies stored in microorganisms, ii) characterization of abnormal accumulations in kidney biopsy, iii) penetration of nanocarriers in tissue or model cell lines, iv) investigations around protein nano-assemblies structure. New experimental approaches were therefore developed to solve the problems encountered during those studies and to overcome the technical constraints imposed by the system itself (configuration of illumination - sensitivity -study of soft sample...). Discussion on issues, challenges and potentials of correlative measurements will be proposed.


Dr. Ariane Deniset-Besseau

In 2008, Dr. Ariane Deniset-Besseau got her Ph.D. degree from University Paris-Sud, France (“Time-resolved 3D imaging as a support for medical diagnosis: development of multifocal multiphoton fluorescence microscope”, supervisor Dr. Fontaine-Aupart). From 2008 to 2009 she served a Post-doctoral position from Laboratory for Optics & Biosciences, Ecole Polytechnique, France (« Second Harmonic Generation of collagen and imaging of the remodelling of the extracellular matrix »)

In 2009, she received a position of Associate Professor in the group of Pr. Alexandre Dazzi at the Université Paris-Sud, France.

Since 2011, she is in charge of soft matter and biological applications in Pr. Alexandre Dazzi’s research group.