Photothermal AFM-IR Spectroscopy and Imaging for Life Science

Versatile Solution for Life Sciences Applications

Photothermal AFM-IR as a Versatile Solution for Life Sciences Applications

Learn more about the diverse applications of photothermal AFM-IR in life sciences, for samples from tissue to single protein fibrils. 

Webinar Summary

Photothermal AFM-IR can provide nanoscale chemical information with highly resolved IR spectra, that directly correlate to FTIR transmission spectroscopy. This webinar covers numerous AFM-IR applications in the field of life sciences, illustrating broad applicability for samples from tissue to single protein fibrils. The speaker also discusses issues, challenges, and potentials of correlative measurements.

Some of the samples and applications presented include:

  • Lipids bodies stored in microorganisms
  • Abnormal accumulations in kidney biopsy
  • Penetration of nanocarriers in tissue or model cell lines
  • Protein nano-assemblies structures


The speaker discusses the new experimental approaches developed to solve problems encountered during these studies, and the efforts to overcome technical constraints imposed by the system itself, such as:

  • Configuration of illumination
  • Sensitivity
  • Soft-sample damage

Find out more information about Bruker's solutions for Photothermal AFM-IR Spectroscopy and Imaging:

Guest Speaker

Dr. Ariane Deniset-Besseau

In 2008, Dr. Ariane Deniset-Besseau got her Ph.D. degree from University Paris-Sud, France (“Time-resolved 3D imaging as a support for medical diagnosis: development of multifocal multiphoton fluorescence microscope”, supervisor Dr. Fontaine-Aupart). From 2008 to 2009 she served a Post-doctoral position from Laboratory for Optics & Biosciences, Ecole Polytechnique, France (« Second Harmonic Generation of collagen and imaging of the remodelling of the extracellular matrix »)

In 2009, she received a position of Associate Professor in the group of Pr. Alexandre Dazzi at the Université Paris-Sud, France.

Since 2011, she is in charge of soft matter and biological applications in Pr. Alexandre Dazzi’s research group.