SPR-24 Pro8
High throughput
Array of eight channels/three spot enables 8800 interactions in 24 hours
Tailored for high-content applications
Analytical biosensor for real-time, label-free characterization of molecular interactions
Continuous Flow SPR Analysis
The SPR-24 Pro8 system is a continuous flow biosensor, delivering sample and reagents to the detection sensors as continuously flowing streams. When sample or reagent injection stops, a “continuous flow buffer” is passed over the sensors.
The formulation of the continuous flow buffer in SPR assays can have a significant impact on the molecular interactions being measured. In many applications, measuring target interactions in a variety of buffer environments is required.
8-buffer feature
The parallel 8-buffer capability of the Bruker SPR-24 Pro8 instruments delivers a substantial enhancement in analytical throughput and experimental flexibility, addressing key demands of modern drug discovery workflows, such as
- High-Throughput and Multiplexed Data Generation
- Acceleration of Pre-Clinical Development
- Enablement for AI-Driven Drug Discovery
It supports condition-dependent analysis, including different pH conditions, salt, detergent, or solvent concentration. Simultaneous multi-buffer analysis saves time and reagends, while increasing information throughput.
Maximising performance with individual needle control
The individual needle control (INC) feature in the SPR-24 Pro8 system allows the eight sample pickup needles to be operated independently as well as in unison. Researchers have the flexibility to choose any combination of 1 – 8 needles to design their optimal assay.
Individual needle control maximizes the performance, flexibility, and scope of applications with the SPR-24 Pro8, while also saving time, materials, and resources when throughput is not the main requirement.
Throughput on demand
The SPR-24 Pro8 delivers faster throughput across a wide range of applications, from kinetic assays with a different target on each sensor to detailed analysis employing multiple in-line controls.
The system uses Bruker’s Hydrodynamic Isolation (HI) continuous flow microfluidic technology to address samples as flowing streams onto the 24 spot SPR+ sensor array. When integrated with an optional plate handling robot, the SPR-24 Pro8 instrument can assay more than 4400 samples per day, generating more than 8800 control subtracted binding responses.
SPR-24 Pro8 can be easily integrated into an automated scheduling software via API.
Throughput with performance
The flexible SPR-24 Pro8 system is ideal for high throughput applications. It offers 24 detection spots, which are addressed by 8 injection needles. Individual needle control (INC) gives researchers the option to choose any combination of 1 to 8 needles, to optimize the performance, flexibility and scope of applications.
The 24-sensor detection array is laid out in 8 flow cells, each containing 3 sensors. Up to 8 samples can be analyzed simultaneously in 8 different buffers, generating up to 24 binding measurements per analysis cycle. All 24 sensors can also be addressed fully independently of the others, providing maximum assay design flexibility.
The versatile system can also save time and materials in the lab, particularly if throughput is not the main requirement.
Up to eight buffers can be used simultaneously in the instrument. The ability to evaluate different pHs, ionic strength, detergent or solvent concentration, as well as the effect of co-factors or inhibitors, in a single assay results in simpler, optimized assays that ultimately increase throughput and productivity.
Enhanced productivity
The design of SPR-24 Pro8 maximizes control analysis flexibility, enabling high throughput for the most complex analyses. Samples can be simultaneously assayed on two controls as well as on the active sensor.
Early analysis of non-specific binding to matrix proteins such as HSA or BSA can be critical. Additional in-line control not only halves the number of assay cycles required but can also improve data quality by eliminating potential variations between multiple sample preparations.
From initial assay development to pre-defined method templates, the Sierra SPR Control Software provides the perfect solution for every use case. It combines the flexibility of the hardware with an easy-to-use drag and drop method editor. A well-defined plate and method library supports personal workflow structuring and the data view allows real-time visual monitoring of flow cells.
Applications of Surface Plasmon Resonance
Tackling Challenging Targets with High-Throughput Biophysical Screening at Novartis
Learn in this note how multiplexing, automation and automated data analysis with Genedata Screener take SPR to the next level in this real-life example.
How to influence ligand density using amine coupling
Finding the ideal immobilization level to obtain optimal kinetic measurements is critical. Here we examine the influencing parameters on different example targets.
Screening and characterization of small molecule binding to protein targets
Real-time, label-free (RT-LF) analysis by SPR detection is a powerful tool for the biophysical characterization of small molecule drugs and drug candidates.
Screening and characterization of biotherapeutics
Real-time, label-free (RT-LF) analysis by SPR detection is a powerful tool for the biophysical characterization of protein therapeutics.
For Research Use Only. Not for use in clinical diagnostic procedures.