Light-Sheet Microscope Optimized for Long-Term 3D Fluorescence Imaging

The InVi SPIM is the first commercially available inverted light-sheet microscope.

It is perfectly suited for a great variety of samples and applications ranging from cell culture to whole embryos (e.g. Drosophila and Zebrafish).

InVi SPIM Light-Sheet Microscope
InVi SPIM Light-Sheet Microscope

Illumination and Detection

The InVi SPIM can achieve a resolution down to 255 nm in xy, enabling subcellular resolution in living samples free of phototoxic effects.

A Nikon CFI Plan Fluor 10x W 0.3 NA water immersion objective lens projects the light-sheet on the sample. Detection includes a high numerical aperture Nikon CFI Apo 25x W 1.1 NA water immersion objective lens. An additional magnification changer results in 31.3x and 62.5x total magnification for field view and sampling adjustment according to your experimental needs.

Click to enlarge - Illumination and detection concept
InVI SPIM - Illumination and detection concept
Illumination Detection Effective Magnification Field of View Pixel Size Optical Resolution
10x/0.3 NA Nikon 25x/1.1 NA 31.3x
62.5x
420µm
210 µm
208 nm
104 nm
255 nm

Illumination Optics

  • Chromatic correction from 440 to 660 nm
  • Light-sheet generation by beam scanning
  • Flexible light-sheet thickness (2 µm to 6 µm)
  • Nikon CFI Plan Fluor 10x W 0.3 NA water immersion objective lens

 

Detection Optics

  • Nikon CFI Apo 25x W 1.1 NA water immersion objective lens
  • 2 spectral detection channels, each equipped with a fast filter wheel (10 positions and 50 ms switching time between adjacent positions)
  • Filters adapted to the selected laser lines
  • 2 high-speed sCMOS cameras Hamamatsu Orca Flash 4.0 V3
  • Maximum frame rate >80 fps at full frame (2048 × 2048 pixels of 6.5 µm × 6.5 µm size) and up to 500 fps at subframe cropping
  • Peak quantum efficiency (QE): 82% @ 560 nm