Recent Studies in ROS Detection

EPR Detection of Reactive Oxygen and Nitrogen Species (ROS and RNS)

Direct detection of ROS is very difficult or impossible in solution at room temperature due to their very short half-life. Electron paramagnetic resonance (EPR) is the only method for the direct detection of paramagnetic species. Here we have assembled a collection of articles outlining some research being done and the effective role EPR is playing.

Detailed study reveals cancer fighting properties of magnolia extract

Honokiol (HNK) is a promising anticancer agent derived from the bark of an oriental medicinal plant Magnolia officinalis. It has been concluded that HNK treatment triggered cytoprotective autophagy in prostate cancer cells, which can be exploited to increase anticancer effect of this promising natural agent. A cell-permeable spin probe (CMH) was used to detect ROS by EPR in prostate cancer (PC-3) cells. Click here to find out how.

‘Radical’ inhibitor targets tumor oxygen supply

Nitric Oxide (NO) and Reactive oxygen species (ROS) are endogenous regulators of angiogenesis-related events as endothelial cell proliferation and survival, but NO/ROS defect or unbalance contribute to cancers. A novel photoactive inhibitor called NS1 constitutes a promising tool that modulates NO and redox stresses with great potential to control angiogenesis. Click here to see how EPR was used as a tool for detecting nitric oxide and monitoring superoxide level in aorta rings isolated from mice.

Mouse study knocks out popular diabetes theory

Type-2 diabetes mellitus (T2DM), with over 6 million new cases reported per year, currently represents one of the world’s chief economic and health care challenges. Recent studies place altered mitochondrial oxidative phosphorylation (OxPhos) as an underlying genetic element of insulin resistance. Click here to find out how simple is direct measurement of mitochondrial ROS production using EPR-spectroscopy.  

The effect of UV exposure on amyloid plaque aggregation in Alzheimer’s disease

Increasing evidence indicates that abnormal Cu2+ binding to Aβ peptides are responsible for the formation of soluble Aβ oligomers and ROS that play essential roles in AD pathogenesis. UV light exposure pronouncedly enhances cytotoxicity of the chelator-treated and -untreated Cu2+ bound Aβ42 aggregates. Click here to see how EPR is a unique tool to detect ROS generated by the aggregates incubated in darkness and exposed to a controlled UV exposure.

Targeting fibrotic diseases at their source: enzymes

The Nox family of enzymes (Nox1 to -5, Duox1 and -2) and mitochondrial electron transport enzymes are among the major sources of cellular reactive oxygen species (ROS), including superoxide radical anion (O2⨪), hydrogen peroxide (H2O2), and related oxidants. Nox-related inflammatory diseases include acute respiratory disease syndrome, chronic obstructive pulmonary disease, neurodegenerative diseases (including Alzheimer disease, Parkinson disease, and ischemic stroke), and reperfusion injury occurring during transplantation. Hence, there is a need for the development of novel drugs based on new targets. Nox enzymes are a promising drug target, based on mounting evidence in humans and in experimental animal models.

Click here and see how EPR represents a most useful assay to determine Superoxide production in cells.  

The antioxidant efficacy of Qing Huo Yi Hao against diabetes-related oxidative stress

Chinese medicine formula Qing Huo Yi Hao (QHYH) reveals to be a potent antioxidant acting as scavenge of superoxide anions in high glucose-treated endothelial cells.

Click here and see how EPR helped to discover it.

Apoptosis and oxidative stress in ischemic stroke

Extensive research has identified citotoxicity, oxidative stress, inflammation, and cell death (necrosis and apoptosis) as key contributory pathways underlying lesion progression.

Click here and see how EPR contributes to politherapy approach to these diverse injury mechanisms.

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