The SamplePro SPE performs the SPE process with the original sample mixture and generates typically one or two fractions per incoming sample. The major components (typically polar like sugars in beverages or endogenous metabolites from bodyfluids) are not retained and are collected in the aqueous load fraction.
The compounds of interest (typically unpolar) are retained on the cartridge, enriched, dried and eluted as concentrated solution with an organic, NMR friendly solvent.
For ion exchange SPE applications, a third fraction is generated by flushing the cartridge with a different SPE solvent containing an acid or base according to the ions to be released from the sorbent.
The complete process of preparation, loading, drying, elution and transfer of the fractions into NMR tubes is fully automated and can process up to 48 samples yielding 96 fractions in an unattended process.
A standard method is provided that is optimized and validated by BRUKER for NMR screening. Quality and reproducibility is essential as small changes in water content, pH value or salt content may considerably affect position and shape of NMR signals and make the evaluation of the data difficult or impossible.