S2 PICOFOX, TXRF Spectrometer, Trace Analysis

Sample Preparation of Tissue, Multi-Phase Mixtures and Similar

Fast dilution procedure

  • Dilute 500 μl of the sample (homogenate) with 0.2 Vol.-% PVA water (1:1)
  • Add 10 μl IS solution (Ga, 50 mg/L, end conc. 0.5 mg/L) and homogenize
  • Pipette 10 μl on a siliconized sample carrier and dry by heat or air

Digestion procedure

  • Add 100 μl conc. nitric acid to 400 μl sample and heat to 70 °C for 1 hour
  • After cooling add 10 μl IS solution (Ga or Y, 25 mg/L, end conc. 0.5 mg/L) and homogenize
  • Pipette 10 μl on a siliconized sample carrier and dry by heat or air

Remarks

  • Alternatively a fast disruption procedure can be applied to tissue:

    • Treat tissue in buffer in an ultrasonic bath
    • Separate particles by centrifugation
    • Add IS to supernatant and transfer to disc

TXRF benefits

  • Fast sample preparation by a simple dilution step possible;
    improved detection limits after treatment with nitric acid
  • High linear range from 50 ppb to 5 %
s2_picofox_wheat.jpg
Samples: wheat
s2_picofox_LLD_fish.jpg
Detection limits for fish standards prepared
as suspension or after digestion
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