JPK CellHesion 生物型原子力显微镜
亮点
CellHesion® 300
The automated CellHesion® 300 is the ideal tool for measuring cell-cell, cell-tissue, and cell-substrate interactions with single-molecule sensitivity. It enables fast and easy measurement of the structure, morphology, and nanomechanical properties of living biological systems, delivering crucial insights into the role they play in various pathological disorders. This innovative system creates novel possibilities for applications in biophysics, biochemistry, implant research, wound healing, developmental biology, stem cell research, infection biology and immune response studies.
特点
Understanding Biomechanics Made Easy
CellHesion 300 provides reproducible, quantitative data of unprecedented quality. Its high degree of automation increases throughput and delivers the productivity, performance, and statistical significance required for biomedical and clinical research environments. Important parameters, such as maximum adhesion force, individual unbinding events, and tether characteristics are automatically determined from each dataset by innovative software solutions.
Only CellHesion 300 delivers:
- Maximized throughput for higher productivity by automating measurements
- Correlative and label-free multiparametric, nanomechanical measurements of living samples in near-physiological conditions
- Fast and simple selection of regions of interest over large sample areas, ideal for tissue biopsies
- Systematic workflow with visual support
"Automatic mechanical screening of highly topographic samples with the CellHesion 300 is of great advantage for tissue characterization in the clinical context."
Prof. Dr. Ansgar Petersen
BIH, Center for Regenerative Therapies
Charité Medical University, Berlin, Germany
Performing Automated Multi-Parametric Nanomechanical Mapping
State-of-the-art software, intuitive user guidance, and automated alignment of the detection system provide the key to autonomous operation and fast results.
The new CellHesion 300 user interface gives the operator quick and easy control of the system and its operating parameters.
The software offers an easy-to-use scripting tool for user-defined experiments. Powerful batch data processing capabilities enable the analysis and quantification of large datasets at the touch of a button.
Discover the Possibilities
Mapping of the viscoelastic properties of samples with a large topography range, over an extended frequency scale with an optional Z-scanner and NestedScanner capabilities
Studying living cells under near-physiological conditions with an extensive range of accessories for control of environmental conditions, such as temperature and CO2 levels
Automate Investigation of Large Samples
The new SmartMapping feature enables the selection of flexible, user-defined 2D shaped force maps. The optimal range of force acquisition is continuously evaluated and automatically adjusted by new large-scaled Z-motors. Using optical tiling, multiple areas of interest can be selected in advance and automatically examined, allowing the easy and effective study of large sample areas. The improved motorized stage accuracy delivers a degree of precision and velocity second to none.
Integrate Seamlessly with Optical Microscopes
CellHesion 300 can be seamlessly integrated into the latest research grade optical microscopes with advanced and super-resolution capabilities, to deliver real-time, correlative data sets for the comprehensive characterization of living biological samples. Versatile experimental setups and automated adjustment
of system parameters opens new possibilities for long-term, self-regulating experiment series. CellHesion 300 is the ideal solution for the investigation of rough surfaces, densely packed cell layers, and highly corrugated tissue samples.
Selection of Scientific Publications Using the CellHesion Technology
- Abuhattum et al., Adipose cells and tissues soften with lipid accumulation while in diabetes adipose tissue stiffens. Sci Rep 12, 10325 (2022).
- Michael et al., Measuring the elastic modulus of soft culture surfaces and three-dimensional hydrogels using atomic force microscopy. Nat Protoc 16, 2418–2449 (2021).
- Liebsch et al., Quantification of heparin’s antimetastatic effect by single-cell force spectroscopy. J Mol Recognit. 34, e2854 (2021).
- Möllmert et al., Zebrafish Spinal Cord Repair Is Accompanied by Transient Tissue Stiffening. Biophys J. 118(2), 448-463 (2020).
- Shen et al., Reduction of Liver Metastasis Stiffness Improves Response to Bevacizumab in Metastatic Colorectal Cancer. Cancer Cell 37(6), 800-817 (2020).
- Rheinlaender et al., Cortical cell stiffness is independent of substrate mechanics, Nat. Mater. 19, 1019–1025 (2020).
- Aaron at al., Quantification of heparin's antimetastatic effect by single-cell force spectroscopy, J Mol Recognit., 1–11 (2020).
- Krieg et al., Atomic force microscopy- based Mechanobiology, Nature Reviews Physics 1, 41–57 (2019)
- Stylianou et al., Review Article: Atomic Force Microscopy on Biological Materials Related to Pathological Conditions, Andreas, Scanning, 8452851 (2019)
- Thompson et al., Rapid changes in tissue mechanics regulate cell behaviour in the developing embryonic brain. eLife 8:e39356 (2019)
- Miroshnikova et al., Adhesion forces and cortical tension couple cell proliferation and differentiation to drive epidermal stratification, Nat Cell Biol 20, 69–80 (2018)
- Elias et al., Tissue stiffening coordinates morphogenesis by triggering collective cell migration in vivo, Nature 554, 523-527 (2018)
- Bharadwaj et al., aV-class integrins exert dual roles on a5b1 integrins to strengthen adhesion to fibronectin, Nature Communications 8, 14348, 1-10 (2017)
- Friedrichs et al., A practical guide to quantify cell adhesion using single-cell force spectroscopy. Methods (2013)
Perfect environment for cells and tissues
CellHesion® 200 is tailor-made for the specialized requirements of working with living cells. The use of standard substrates such as 35mm Petri dishes with or without glass bottom or round coverslips makes cell cultivation and handling easy.
Temperature control from 15°C to 60 °C, fluid exchange and ports for CO2 control are integrated in the JPK PetriDishHeater™ or in the JPK BioCell™ designed for coverslips. As an option, the CellHesion® 200 can be integrated in existing incubators (ask for model).
All parts of the setup which are in contact with the sample can be sterilized. CellHesion® 200 offers enough vertical-axis travel range to handle large cells and separate even well-adhering cells from their substrates.
Application examples
- Stiffness and elasticity mapping from single cells to substrates and tissues
- Cell-cell and cell-substrate interactions
- Cell adhesion and tether formation
- Automated mapping of sample properties over a large range for structured substrates, microspheres, cells. etc. with the new HybridStage™
- Biomaterial studies, biofouling, biosensors, capsules
- Implants coatings and cellular biochips
- Applications in microbiology and virus research
- Pharmaceutical studies such as drug delivery mechanisms
- Applications in food, paper or textile industry on fibers, coatings or powders in air or liquid
- Binding studies such as receptor/ligand or antibody/antigene
- Testing functionalized surfaces
Key Features
- Innovative platform for cell or tissue mechanics and adhesion experiments
- Characterization of cell/cell or cell/substrate interaction, cell elasticity, tether formation, adhesion, and cellular response
- Quantitative measurements from single molecules to entire cells and tissue, organelles and embryos with highest precision
- Automated mapping of sample properties over a large range for structured substrates, microspheres, cells. etc. with the new HybridStage™
- Intuitive user interface with ExperimentPlanner™ for perfect workflow
- Integrates with advanced optical imaging such as DIC, phase contrast, fluorescence, confocal microscopy, Superresolution, TIRF, FRET, FLIM, etc.
- Widest range of modes and accessories
应用
CellHesion® 200 Data Gallery
Bruker’s BioAFMs allow life science and biophysics researchers to further their investigations in the fields of cell mechanics and adhesion, mechanobiology, cell-cell and cell-surface interactions, cell dynamics, and cell morphology. We have collected a gallery of images demonstrating a few of these applications.
技术参数
Specifications
- Innovative platform for cell adhesion/cell mechanics research for measurements from single-molecules to entire cells and tissue
- Compatible with inverted microscopes for combined experiments, e.g., Zeiss Axio Observer, Axiovert A1, Nikon Ti/TE 2000, Nikon Ti2, Olympus IX series and Leica DMi series
- Compatible with all major light microscopy techniques such as DIC and phase contrast or fluorescence and confocal techniques such as TIRF, FRAP, LSM and others
- Compatible with upright macroscopes and stereo microscopes such as Zeiss AxioZoom V.16, Leica Z16 ApoA, Leica M205FA and Olympus MVX10
- Automated range adaption in z direction for strong corrugated surfaces
- Cantilever sensor lifting system with >110µm travel range with closed-loop control through high-speed capacitive sensor feedback
- Equipped with motorized precision stage, in particular for tissue probing, within 20×20mm range
- Living cell studies in native environment with tempeature control, perfusion and gas flow (CO2) on coverslips with the JPK BioCell™ or 35mm Petri dishes with or without glass bottom from Wilco, BD, WPI with the JPK PetriDishHeater™
- All parts which came in contact with the sample are easy to clean
- Compatible with standard incubators (specify model)
- High-throughput determination of cellular interaction parameters through JPK software batch processing
- Single-molecule events, work of removal, maximum adhesion forces, number of unbinding events and viscoelastic parameters such as Young’s modulus in one system
配件
The Widest Range of Accessories in the Market
Optical systems/accessories, electrochemistry solutions, electrical sample characterization, environmental control options, software modules, temperature control, acoustic and vibration isolation solutions and more. Bruker provides you with the right accessories to control your sample conditions and to perform successful experiments.
网络研讨会
Watch Recent BioAFM Webinars
Our webinars cover best practices, introduce new products, provide quick solutions to tricky questions, and offer ideas for new applications, modes, or techniques.