Luxendo Light-Sheet Microscopes

LCS SPIM

The large cleared sample light-sheet microscope
LCS SPIM (Large Cleared Sample Light-Sheet Microscope)

Highlights

Two-sided illumination, single detection, high-throughput imaging

LCS SPIM

The LCS SPIM is a compact light-sheet microscope designed for very fast and very gentle 3D imaging of large optically cleared samples. Like the Luxendo MuVi SPIM CS, it combines the advantages of light-sheet fluorescence microscopy and of tissue clearing methods. Novel and unique solutions facilitate mounting and fast microscopy of much larger samples, rendering it a unique tool in its field. Typical applications of the microscope include imaging of large cleared-tissue samples, e.g. whole mouse brains to visualize complete neuronal and vascular networks. Microscopy of cleared organs or thick tissue samples is highly relevant in developmental biology studies and for cancer research, e.g. when investigating organ development, tumor structure and tumorigenesis.

Compact, Benchtop Design
Sophisticated optics, laser Class 1 Safety standard and small footprint. No necessity of a dedicated microscope room
Large and Closed Cuvette
Cuvettes with up to 6 cm in length and 3.5 cm in width alow imaging of whole, cleared animals.
High-Throughput Capability
Standardized cuvette sample preparation, mounting, and imaging. It allows for high-throughput imaging without direct exposure to toxic chemicals.

Eigenschaften

Compact, Benchtop Design

The LCS SPIM's compact, robust and vibration-free design provides maximal stability even during long-term high-throughput experiments.

The basic configuration of the LCS offers a cost-effective solution for cleared sample imaging. Based on a very versatile layout, the highly flexible upgrade options expand the performance of the system to increase speed and optical performance.

Tailored to fit your lab bench, this class 1 laser system does not require any air table or vibration-compensation mechanism as all moving components are light-weight and balanced. Maximal stability of focus and thermal conditions are also guaranteed. The proprietary piezo-crawler stages ensure longevity and precision for a permanently accurate specimen positioning. Neither the images nor the quality of your sample are affected thanks to the unique and very gentle image-acquisition concept.

Large and Closed Cuvette

An exchangeable quartz-crystal cuvette simplifies the whole sample mounting procedure as well as cleaning and maintenance, while ensuring compatibility with a wide range of clearing solutions by covering a refractive-index (RI) range from 1.33 to 1.57.

The cuvette is available in different lengths and sizes to adapt for samples of different extent: the dimensions of the cuvette range from [20 mm × 20 mm × 20 mm] up to [40 mm x 30 mm x 30 mm] (l × w × h).

 

High-Throughput Capability

A quartz crystal cuvette facilitates the fast mounting of large, delicate samples. The specimen is simply introduced into the cuvette filled with the clearing solution.

The motorized sample stage for positioning and stack acquisition as well as the programmable optics concept for fast 3D scanning of the light-sheet through the sample ensure that the sample remains unperturbed during the imaging experiment while achieving the highest acquisition speed.

Illumination and Detection

The illumination optics comprise light-sheet generation by scanning a beam after passing a beam-shaper unit for length and diameter adjustment. Two Nikon 4x 0.2 NA air objective lenses project two aligned light sheets from opposing directions on the sample. The expandable and upgradable illumination setup allows a very flexible choice of lasers.

The detection optics comprises one Olympus 4x 0.28NA air/dry lens. An additional magnification changer results in a total magnification between 2.2x and 8.8x. The spatial detection arm is equipped with 10 position high-speed filter wheel. The robust sCMOS camera is well-suited for experiments that require high detection efficiency, quantification and speed.

Lux DATA - A Comprehensive Data Processing and Storage Solution

Cutting-edge biological imaging like light-sheet microscopy, but also super-resolution or two-photon imaging, generates a vast amount of data required to provide researchers with all the relevant information about their samples. However, storage, transfer, and processing of the data remain a challenge.

Anwendungen

Cleared-Sample / Cleared-Tissue Applications

Browse a selection of applications data from our customers below. Researchers are using LCS SPIM in a variety of ways including studies.

Spezifikationen

Specifications

Illumination Detection
Effective Magnification
Field of View Pixel Size Optical Resolution
4x / 0.2 NA Olympus 4.0x / 0.28 NA

2.2x

4.4x

8.8x

6600 µm

3300 µm

1515 µm

2.96 µm

1.48 µm

0.74 µm

1.1 nm

Illumination Optics

  • Chromatic correction from 440 to 660 nm
  • Light-sheet generation by beam scanning
  • Flexible light-sheet thickness
  • 2 Nikon 4x 0.2 NA air objective lenses
  • 2 identical illumination arms
  • Optionally: axial beam scanning for an extended FOV
 

 

Detection Optics

  • One Olympus 4x 0.28 NA air/dry objective lens
  • One detection arm equipped with a fast filter wheel (10 positions and 50 ms switching time between adjacent positions)
  • Filters adapted to the selected laser lines
  • One high-speed sCMOS camera
  • Maximum frame rate up to 80 fps at full frame (2048 × 2048 pixels of 6.5 µm × 6.5 µm size) and up to 500 fps at subframe cropping
  • Peak quantum efficiency (QE): 82% @ 560 nm
  • Speed: 5 min for a whole mouse brain (12 mm x 12 mm x 12 mm)
        - 30s per stack
        - 4 stacks
        - Sampling size: 3 μm × 3 μm

Fast Imaging Mode

The basic configuration of the LCS SPIM can be upgraded with axial illumination scanning to extend the field of view (FOV) and to reduce shadowing effects.

 

 

Laser Combiner

  • Maximum of 6 laser wavelengths (405, 445, 488, 515, 532, 561, 594, 642, and 685 nm with 40 mW each before fiber), more options available upon request
  • Fast modulation (MHz range) and high extinction ratio

Software

Software

Luxendo's intuitive user interface offers a simple setup and execution of multidimensional experiments, while real-time control is handled by an embedded controller to ensure microsecond-precision timing independent of the PC’s performance fluctuations.

Precise timing control of all connected devices is a prerequisite for reliable experimental outcomes. Full control of data streaming to storage as well as GPU-supported image processing further complements the overall performance.

Electronics, Microscope Software and Computer

  • Embedded microscope software with an open communication interface: documented API, TCP/IP-based communication
  • Flexible GUI for interactive microscope control and experiment design
  • Computer with 256 GB RAM, Intel dual 8 core CPU
  • High-speed RAID controller for data streaming, 8 × 4 TB local storage in RAID O
  • GeForce RTX 3070 graphics card
  • 2x10 Gbit/s, 2x1 Gbit/s, optional 2x optical port
  • 1 UHTV 40 inch display

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