Neuroscience Virtual Workshop 2021 – Imaging Applications in Neuroscience: Assessing Neuronal Structure and Function

       _{PRESENTATION HIGHLIGHTS:}

• [00:01:25] Why image neuronal activity with two-photon microscopy/GCaMP calcium indicator 
• [00:06:57] Intro to the Ultima 2Pplus two-photon holographic microscope system
• [00:09:24] How to set up a SLM experiment
• [00:10:30] Uncaging galvo calibration
• [00:11:43] How to perform SLM calibration
• [00:18:48] Synchronizing optogenetics  stimulation with calcium imaging
• [00:20:00] Use Cases and Sample Images
• [00:24:00] More about calibration

       _{LIVE AUDIENCE Q&A:}

• [00:22:35] How does the Prairie View software handle calibration of the zero-order block/stop you from selecting those areas to stimulate?
• [00:25:57] What mouse lines are available for simultaneous calcium imaging and optogenetic stimulation?

       _{PRESENTATION HIGHLIGHTS:}

• [00:01:06] Introduction to light-sheet microscopy
• [00:03:26] Review of Bruker's SPIM instruments
• [00:04:24] Features/applications of LCS SPIM
• [00:13:48] Practical Session: How to mount a sample for imaging with LCS SPIM
• [00:19:20] Practical Session: Software and user-interface settings; imaging a sample in real time
• [00:26:05] Live Audience Q&A

       _{LIVE AUDIENCE Q&A:}

• [00:26:12] Are the illumination lenses always the same? Can you think of any reason to change them for different purposes?
• [00:27:11] How large the sample be? Can you scan a whole rat brain?
• [00:27:51] What is the resolution and field of view of the LCS SPIM instrument? 

       _{PRESENTATION HIGHLIGHTS:}

• [00:01:45] Intro to super-resolution and Single-Molecule Localization Microscopy (SMLM)
• [00:04:48] Why and how super-resolution microscopy was developed
• [00:10:36] SMLM methods and applications
• [00:14:14] How Bruker's Vutara VXL SML microscope achieves even higher resolution
• [00:15:58] Use Cases and Sample Images: SMLM in neuroscience research applications
• [00:17:56] Review of the capabilities and instrumentation of Bruker's Vutara VXL
• [00:20:30] Practical Session: SML imaging of microtubulin, performed in real time
• [00:24:38] Rendering and animating data with Bruker's SRX software

       _{LIVE AUDIENCE Q&A:}

• [00:29:16] How difficult or easy is sample preparation for SMLM?

       _{PRESENTATION HIGHLIGHTS:}

• [00:00:17] What is ChipCytometry?
• [00:01:27] Review of traditional methods for studying protein biomarkers and how ChipCytometry combines their best capabilities
• [00:04:24] Basic/standard workflows and setup
• [00:07:40] 14-plex Neuro Panel overview
• [00:12:35] Use Cases and Sample Images: Cholinergic neurons, glutamatergic and dopanimergic receptors, and microglia markers
• [00:17:41] Practical Session: How to generate quantitative, single-cell analyses with the ChipCytometry platform

       _{LIVE AUDIENCE Q&A:}

• [00:23:30] What is the maximum plex, in total, that can be run on ZellScannerONE?
• [00:24:09] Is the Neuro Panel limited to mouse or is it usable in applications with other species?