timsTOF Ultra 2

Unleash 4D-Proteomics™ with the timsTOF Ultra 2 – the ultimate fusion of trapped ion mobility spectrometry (TIMS) with quadrupole time-of-flight (QTOF) technology.

Redefining sensitivity

 From single cells to global impact

Triumph in proteomics with speed and superior resolution

Ultra sensitivity
TIMS PASEF® mode parallelization captures ions with near 100% efficiency, delivering exceptional sensitivity and unparalleled proteome depth at 300 Hz speeds.
Maximum ion transfer
Optimized CaptiveSpray Ultra 2 (CSI Ultra 2) ionization creates a vortex for optimal ion transfer and stable spray without adjustment. 
Enhanced specificity
MOMA (Mobility Offset, Mass Aligned) allows identification and separation of co-eluting isobaric mass-to-charge ions in the mobility domain, increasing clarity and confidence.
Increased confidence
Confidence with low S/N peptide identification derived from CCS-Enabled TIMScore™, TIMS DIA-NN 4D-Proteomics™ or using Spectronaut® 19.

Upgrade your research platform for continuous innovation

We recognize the inherent duality of research investments: opportunity and risk. Embracing innovation's dynamism, we prioritize upgradable research platforms. This commitment fosters sustainability and empowers researchers to navigate the ever-evolving landscape.

Our upgradable platform seamlessly integrates the latest advancements, keeping your projects at the forefront of discovery. It grants researchers the flexibility to choose the optimal moment to leverage these advancements, maximizing the impact of their work and aligning perfectly with their research goals.

Your path to upgrading your timsTOF

“I appreciate that timsTOFs are field-upgradable, allowing my team to access the next gen MS performance without replacing the entire system” 

Jenny van Eyk, Ph.D., Director of Basic Science Research in the Barbra Streisand Women’s Heart Center and Professor of Cardiology at Cedars-Sinai, Los Angeles, CA, USA

Unmatched sensitivity for groundbreaking omics research

Introducing the next generation of timsTOF Ultra mass spectrometry: the timsTOF Ultra 2. Paired with the powerful CaptiveSpray Ultra 2 ion source, this dynamic duo unlocks unparalleled sensitivity, empowering you to conquer challenging samples and detect low-abundance peptides. This translates to groundbreaking discoveries in omics research.

Dive Deeper: From minute samples to unseen peptides
timsTOF Ultra 2 and CaptiveSpray Ultra 2 join forces to reveal the full potential of your samples. Analyze even the smallest amounts and uncover previously unseen peptides. PASEF® acquisition empowers you to delve into the depths of low-abundance peptides, extracting crucial information that was once undetectable. Additionally, trapped ion mobility separation eliminates the need for chemical tagging, streamlining the identification of novel peptides. With these advancements, the timsTOF Ultra 2 with CaptiveSpray Ultra 2 redefine what's possible in your lab, propelling your research to new heights.

The timsTOF Ultra 2 transcends conventional limitations, opening doors to new scientific possibilities. Whether it's single cell proteomics, immunopeptidomics, or plasma proteomics, the timsTOF Ultra 2 with CaptiveSpray Ultra 2 delivers the speed and sensitivity researchers need to push the boundaries and achieve groundbreaking results.

Deeper protein identification with Bruker ProteoScape™

Maximize your identification depth and throughput with the new capabilities of Bruker ProteoScape™, our Run & Done database search platform. TIMScore™ and TIMS DIA-NN leverages the power of the CCS for more accurate protein identification on the fly. Additionally, Bruker ProteoScape™ provides seamless integration with Spectronaut® 19, granting access to DirectDIA+ and enabling powerful diagonal-PASEF workflows.

The timsTOF Ultra 2: Unmatched performance for deep proteome analysis

The second generation of timsTOFs is all about increasing sensitivity without compromising robustness. Using the CSI Ultra 2 with its optimized vortex dynamics as well as access to diagonal-PASEF® workflows and the second generation of ion charge control (ICC 2), the timsTOF Ultra 2 pushes the boundaries of sensitivity even further.

Experience the power of timsTOF Ultra 2
With the timsTOF Ultra 2, robust and highly sensitive mass spectrometry becomes your indispensable tool for exploring phenotypes within heterogeneous cell environments. Unravel the secrets of cancers and shed light on other diseases with complementary information to single cell genomics and transcriptomics. Decipher heterogeneity to deepen your understanding of biological processes, cell-cell communication, and enhance therapeutic options.

Discover a new era of single cell proteomics
Unleash the full potential of single cell proteomics with timsTOF Ultra 2. Peer into cellular complexity like never before. Gain a comprehensive understanding of unique phenotypes within diverse cell populations, and revolutionize the perception of diseases and their underlying mechanisms.

Illuminate the path to progress
Propel your research by harnessing the power of advanced mass spectrometry, and drive transformative breakthroughs in proteomics with the timsTOF Ultra 2. Unlock cellular mysteries, transcend conventional understanding, and shape a future where improved therapies become a reality.

Witness cells in a whole new light. Revolutionize your approach to proteomics and unlock the secrets of cellular complexity for a brighter tomorrow.

A powerhouse for molecular medicine: tackling single cell analysis in FFPE tissue

Looking to elevate your single cell analysis? Take on the challenge of analyzing single cells from FFPE tissue.

Discover the power of timsTOF Ultra in the realm of translational patient treatment. With its exceptional capabilities, timsTOF Ultra enables the precise mapping of FFPE tissue proteins to specific cancer regions, even with limited sample quantities. Experience the next-level performance of dia-PASEF® on the timsTOF Ultra, revolutionizing low-input tissue proteomics and becoming an essential solution for molecular medicine labs.

Unleash the potential of molecular medicine with timsTOF Ultra as your ally. Conquer the complexities of single cell analysis in FFPE tissue and embark on a transformative journey towards advancing patient care.

The analysis of protein group and precursor identification rates in FFPE mouse liver tissue, dissected by laser capture microdissection, with varying cell equivalents, demonstrating increased protein group abundance and correlation of protein abundance between different cell equivalents (data from timsTOF Ultra).

"The single-cell sensitivity dia-PASEF® workflow on the timsTOF Ultra has brought our low-input tissue proteomics work to a new level. Using a 20-min nanoflow LC gradient combined with Bruker’s optimized dia-PASEF® (3x8 window) method, we can reproducibly quantify 1,500-2,000 proteins from laser microdissected mouse liver FFPE tissue of only 1,500 μm2, regions of approximately 1-2 hepatocytes. Due to its great sensitivity and robustness, the timsTOF Ultra is our main workhorse in the lab."

Fabian Coscia, Ph.D., Group Leader Spatial Proteomics, Max Delbrück Center for Molecular Medicine, Berlin, Germany 

Unleashing the potential of TIMS and PASEF®-based metaproteomics in microbiome research

Microbial communities play a crucial role in planetary health, impacting the well-being of humans, animals, and plants, and driving interventions for acute and global issues. As microbiome research expands and influences legislation, economics, and societal behavior, it is essential to go beyond taxonomical characterization and delve into the functional dynamics and environmental interactions of these communities. Metaproteomics has emerged as a powerful approach to gaining this understanding.

Discover the advantages:

  • Unparalleled peptide and protein identification: Utilize the ion mobility dimension of TIMS and PASEF® to identify and quantify an unprecedented number of peptides and proteins in the mouse gut microbiome and host with exceptional accuracy (CV <20%).
  • Comprehensive taxonomical and functional profiling: Achieve profiling depths that rival or surpass commonly used genomic approaches, enabling comprehensive insights into both the taxonomical and functional aspects of microbial communities.
  • Sensitivity and efficiency: Experience remarkable sensitivity gains, allowing analysis with minimal sample amounts, while accelerated analysis using short gradients is made possible by the incredible speed of PASEF®.
(A) Mouse feces samples were measured on the timsTOF Ultra with varying injection amounts and gradient lengths. Data was analyzed to show the total number of identified species as well as KEGG Pathways. (B) 5 orders of magnitude sensitivity with very high reproducibility throughout. Data generated and analyzed by Feng Xian, Christoph Krisp and David Gómez-Varela.

"Metaproteomics is a new tool in microbiome research with the unique potential to go beyond taxonomical characterization and reach a deep understanding of the functional alterations in microbial ecosystems. The analysis of the immense peptidome landscape of microbial samples is an enormous challenge.


The timsTOF Ultra enabled us to reach unprecedented taxonomical identification levels (similar to most genomic approaches) and to improve several fold the quantification, sensitivity, and high-throughput levels of state-of-the-art proteomic technologies. I believe that the use of the timsTOF Ultra will mark a new era in microbiome research, paving the way for exciting findings across biotechnology fields."

David Gómez-Varela, Ph.D., Systems Biology of Pain, Division of Pharmacology and Toxicology, University of Vienna, Vienna, Austria

Advancing plasma proteomics with higher flow rates

Plasma proteomics plays a vital role in disease diagnosis and therapy, offering a comprehensive view of the proteome. To enable reliable identification and monitoring of biomarkers in large cohort studies, it is essential to leverage population-wide screening technologies. The integration of robust µ-flow and normal flow chromatography with timsTOF Ultra 2, powered by VIP-HESI, provides an unparalleled combination of robustness, speed, reproducibility, sensitivity, and sample throughput.

Key features:

  • Robust electrospray technology enables up to 5000 LC/MS runs, providing exceptional longevity.
  • Achieve 3x signal gain at a flow rate of 50 µL/min with the 50 µm sprayer, ensuring optimal sensitivity.
  • Effortlessly replace the standard VIP-HESI sprayer with a convenient drop-in replacement for seamless integration.
  • Experience efficient peptide detection and quantitation in under 10 minutes per sample with our 4D-Proteomics™ approach.
  • Benefit from increased column lifetime, reducing maintenance and replacement costs.

Elevate your plasma proteomics research with our advanced solution, optimized for higher flow rates. Benefit from exceptional robustness, sensitivity, and throughput, empowering you to unravel the complexities of plasma proteomes and accelerate discoveries in disease diagnosis and therapy.

Ultra-sensitive cellular lipidomics for unprecedented insights

The diagnostic potential of lipids and their crucial role in cellular processes have sparked a demand for next-generation sensitivity in lipidomics. Analyzing lipids at the cellular level enables detailed insights into cell heterogeneity, paving the way for breakthrough discoveries in single-cell biology, cancer pathology, and clinical research.

Experience unmatched analytical depth with the exceptional sensitivity of the timsTOF Ultra, combined with nano-flow chromatography using the Bruker nanoElute® 2 and CaptiveSpray Ultra source. This powerful combination empowers researchers to delve into the intricate world of cellular lipidomics and unravel the complex relationships between lipids and cellular functions.


  • Comprehensive MS/MS coverage: Benefit from comprehensive MS/MS coverage from single injections using PASEF® acquisition, ensuring thorough exploration of lipid profiles.
  • Improved MS/MS spectral quality: Achieve superior MS/MS spectral quality through MOMA separation, enhancing the accuracy and reliability of lipid annotations.
  • Increased confidence with accurate CCS Values: Leverage the accurate Collision Cross Section (CCS) values obtained from TIMS, enhancing confidence in lipid identification and characterization.

Unlock the potential of cellular lipidomics with our ultra-sensitive solution. Gain unprecedented insights into lipid composition and function, revolutionizing your research in diverse fields such as single-cell biology, cancer pathology, and clinical investigations. Elevate your lipidomics studies to new heights of sensitivity and precision with the timsTOF Ultra and MetaboScape® and pave the way for transformative discoveries in lipid-related research.

Use the timsTOF Ultra in combination with the nanoElute® 2 to dig into single cell lipidomics. Complement your proteomics results for more complete characterization.

timsTOF Ultra 2 related information