High-performance liquid chromatography (HPLC) reversed phase columns are crucial components that determine the degree of separation in every analysis. In the world of proteomics, unlocking separation with precision can help with the quality of your analysis.
Scientists, researchers, and clinicians desire robustness, speed, and sensitivity from their HPLC and mass spectrometry (MS) systems. Having a system that can accommodate large sample cohorts with minimal maintenance during chromatographic separation is a desirable trait to ensure sample reproducibility. The key to optimizing the chromatography process starts with columns. Having the right chromatographic column will assist in accurate data collection, reproducible results while offering higher throughput, and sensitivity.
This is where columns and tips are critical:
(a) Bridging the gap between liquid chromatography (LC) and MS
(b) Retaining peptides/ binding to active sites
(c) Chromatographic resolution
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PepSep™ C18 columns are the preferred columns in academic and core labs that are focused on speed, reproducibility and sensitivity. Easily connect columns to nanospray sources with a simple twist on and twist off design.
PepSep™ HPLC columns are perfect for all proteomics applications. These columns are designed for high-throughput applications and offer superior sensitivity and resolution. With a diverse portfolio of nanoflow columns featuring various lengths, inner diameters, and particle sizes, rest assured with industry leading materials and connections to accommodate all chromatographic separation needs.
Spend less time swapping columns and more time on the important things, results. Amplify your experience when you combine PepSep™ consumables with timsTOF series instruments, powered by Bruker ProteoScape™ to enhance your ability to acquire data at PASEF® speeds.
PepSep™ ULTRA XL
Ultra power for spectral library generation, designed for longer gradients.
PepSep™ MAX HT
Maximize speed, resolution and dynamic range.
Ultra-high sensitivity and reproducibility at PASEF® speeds
Exceed sample throughput limits at PASEF® speeds with high 4D-sensitivity
Maximize performance to accurately analyze 1000s of samples
The PepSep™ ULTRA XL column (50 cm x 75 µm x 1.5 µm) is designed for long gradients, which are essential for spectral library generation. Spectral libraries play a crucial role in proteomics, particularly in mass spectrometry-based proteomics.
Choosing the right column is difficult. A make-or-break decision that may increase protein and peptide identifications. The PepSep™ ULTRA (25 cm x 75 µm x 1.5 µm) C18 HPLC columns enable the analysis of a higher number of proteins and peptides from routine to restricted sample types.
Designed for high-throughput proteomics, the PepSep™ MAX HT (5 cm x 150 µm x 1.5 µm) allows for gradients less than 15 minutes. This makes it ideal for applications where speed is essential, such as the analysis of clinical samples or the screening of drug libraries.
Scientists spend time fine tuning their methods to achieve broader coverage and higher throughput when mining plasma proteomes.
The PepSep™ MAX (10 cm x 150 µm x 1.5 µm) increases instrument performance to help drive your analysis. When combined with the timsTOF HT, immerse yourself in 4D-Proteomics™, so you can accurately analyze 1000s of samples with high throughput at PASEF® speed.
The proteomics landscape requires high throughput to capture meaningful rich data. PepSep™ MAX columns help you get there. It's time to improve your HPLCs capabilities.
Identify, characterize and quantify peptides and proteins. Get excellent reproducibility, while outlasting leading columns up to 2x longer.
"At first, we were just using PepSep™ columns on our Bruker timsTOF Pro mass spectrometer. But we have been so impressed with their performance and affordable price point that we now use them across all instruments in our lab.
These columns really hit a sweet spot of top-notch chromatography but with minimal back pressure. When compared to traditional nanospray columns, this allows us to use higher flow rates and minimize LC overhead time. This has been crucial for throughput, particularly when thousands of samples using shorter gradients."
Core labs and academic labs want a single column solution for all applications to achieve deeper coverage, higher throughput, and better identification.
PepSep™ XTREME (25 cm x 150 µm x 1.5 µm) will help increase instrument performance, allowing scientists the liberty to have both sample throughput at PASEF® speeds and high 4D-sensitivity to dive deeper into the unknown and open the potential of the plasma proteome.
Rather than deciding between a shorter gradient and high throughput, PepSep™ XTREME C18 HPLC columns offer you the best of both worlds. It’s time to improve your proteomics analysis with better coverage, throughput, sensitivity, and reproducibility. Pair the PepSep™ XTREME with the timsTOF Pro 2 to find unprecedented proteomics depths — more than 5500 protein groups, 42,000 peptides in a 30 min gradient.
Plug and play ready-to-use emitters are equipped with column connection union and spray tip to facilitate a hassle-free Captive Spray setup. With two IDs available, PepSep™ emitters cover the unique demands of proteomics applications.
For Research Use Only. Not for use in clinical diagnostic procedures.