HPLC & LC-MS CONSUMABLES

PepSep Columns & Emitters

Everything you need for PASEF® speed

Introduction

PEPSEP COLUMNS & EMITTERS

Mega-sensitivity, High Throughput, and Maximum Reproducibility

High-performance liquid chromatography (HPLC) reversed phase columns are crucial components that determine the degree of separation. In the world of proteomics, unlocking separation with precision can help with the quality of your analysis. 


Scientists, researchers, and clinicians desire robustness, speed, and sensitivity from their HPLC and mass spectrometry (MS) systems. This is when having a system that can accommodate large sample cohorts with minimal maintenance during chromatographic separation. The key to optimizing the process starts with columns. Having the right chromatographic column will assist in accurate data collection, reproducible results while offering higher throughput, and ultra-sensitivity.

 

This is where columns and tips are critical:
   (a) Bridging the gap between liquid chromatography (LC) and MS
   (b) Retaining peptides/ binding to active sites
   (c) Chromatographic resolution
    

Flow with PepSep

PepSep C18 columns are the preferred column in academic and core labs that are focused on speed, reproducibility and sensitivity. Easily connect columns with a simple twist on and twist off design for nanospray.

Spend less time swapping columns and more time on the important things, results. Amplify your experience when you combine PepSep consumables with timsTOF series instruments, powered by PaSER to enhance your ability to acquire data at PASEF® speeds. 

Overview

The Best Column for Your Needs


PepSep MAX
Maximize sensitivity and reproducibility at PASEF® speeds


PepSep XTREME
Exceed sample throughput limits at PASEF® speeds with high 4D-sensitivity


PepSep ULTRA
Ultimate performance to accurately analyze 1000s of samples

PepSep MAX

Maximize Sensitivity

Based on the results from different digest concentrations of K562 run on the PepSep MAX column (25 cm x 75 µm x 1.5 µm) over a 60 minute gradient. The PepSep MAX column was coupled with nanoElute and timsTOF HT using a 6x3 Dia-PASEF® window scheme and PaSER to search the data in real-time.

Choosing the right column is difficult. A make-or-break decision that may increase protein and peptide identifications. The PepSep MAX (25 cm x 75 µm x 1.5 µm) C18 HPLC columns enable you to analyze a higher number of proteins and peptides from routine to restricted sample types. 

Ultra-high Sensitivity for Single Cell Proteomics

When paired with the timsTOF SCP, exceed your limits of reproducibility, with maximum sensitivity even at PASEF® speed.

Longer gradients with higher sensitivity and proteome depth are now possible. Identify more than 2000 proteins from 500 pg of cell digest and 1500 proteins identified from 250 pg easily as shown on the right. Discover true sensitivity and uncover proteomic patterns, one cell at a time to take your single cell proteomics to the next level with PepSep MAX.

Proteins identified from a dilution series of HeLa digest ranging from 250 pg to 25 ng of peptide load using the Evosep Whisper method.
Protein distribution according to abundance rank shows a dynamic range of about 4 orders of magnitude.

PepSep XTREME

Greater Depth, Breadth and Rapid Proteomics

Results from PepSep XTREME (25 cm x 150 µm x 1.5 µm) columns on 45- and 60-min gradients with different K562 digest concentrations. PepSep XTREME was coupled with nanElute and timsTOF HT using a 6x3 dia-PASEF® window scheme and PaSER to search the data in real-time.

Core labs and academic labs want a single column solution for all applications to achieve deeper coverage, higher throughput, and better identification. 

PepSep XTREME (25 cm x 150 µm x 1.5 µm) will help increase instrument performance, allowing scientists the liberty to have both sample throughput at PASEF® speeds and high 4D-sensitivity to dive deeper into the unknown and open the potential of the plasma proteome. 

Excellent Performance Driven by Enhanced 4D-Sensitivity 

Rather than deciding between a shorter gradient and high throughput, PepSep XTREME C18 HPLC columns offer you the best of both worlds. It’s time to improve your proteomics analysis with better coverage, throughput, sensitivity, and reproducibility. Pair the PepSep XTREME with the timsTOF Pro 2 to find unprecedented proteomics depths — more than 5500 protein groups, 42,000 peptides in a 30 min gradient.

Using the nanoElute UHPLC coupled to the timsTOF Pro, 200 ng K562 was run on the PepSep XTREME with the 5 mm Thermo Pepmap trap. In this experiment, the default short gradient DDA MS method was used. The data was analyzed using MSFragger.

PepSep ULTRA

High Throughput For All Your Proteomics Workflows 

Scientists spend time fine tuning their methods to achieve broader coverage and higher throughput when mining plasma proteomes. 

The PepSep ULTRA (10 cm x 150 µm x 1.5 µm) increases instrument performance to help drive your analysis. When combined with the timsTOF HT, immerse yourself in 4D-Proteomics™, so you can accurately analyze 1000s of samples with high throughput at PASEF® speed.

Analyze More Samples, Less Time, Better Reproducibility

The proteomics landscape requires high throughput to capture meaningful rich data. PepSep ULTRA columns help you get there. It's time to improve your HPLCs capabilities. 

Identify, characterize and quantify peptides and proteins. Get excellent reproducibility, while outlasting leading columns up to 2x longer.

"At first, we were just using PepSep columns on our Bruker timsTOF Pro mass spectrometer. But we have been so impressed with their performance and affordable price point that we now use them across all instruments in our lab.  

 

These columns really hit a sweet spot of top-notch chromatography but with minimal back pressure. When compared to traditional nanospray columns, this allows us to use higher flow rates and minimize LC overhead time. This has been crucial for throughput, particularly when thousands of samples using shorter gradients."  

Danielle Swaney, Assistant Professor, Cellular Molecular Pharmacology, University of California, San Francisco, USA

Emitters

When Performance Meets Simplicity 

Ready to use pulled fused silica emitter without the hassle. Simple plug and play design. Spend less time worrying about tip setup with a static tip ready to use out of the box.

Available in two internal identifications for high throughput, plasma, or single cell proteomics applications.

More Information

 

For Research Use Only. Not for use in clinical diagnostic procedures.