High-performance liquid chromatography (HPLC) reversed phase columns are crucial components that determine the degree of separation. In the world of proteomics, unlocking separation with precision can help with the quality of your analysis.
Scientists, researchers, and clinicians desire robustness, speed, and sensitivity from their HPLC and mass spectrometry (MS) systems. This is when having a system that can accommodate large sample cohorts with minimal maintenance during chromatographic separation. The key to optimizing the process starts with columns. Having the right chromatographic column will assist in accurate data collection, reproducible results while offering higher throughput, and ultra-sensitivity.
This is where columns and tips are critical:
(a) Bridging the gap between liquid chromatography (LC) and MS
(b) Retaining peptides/ binding to active sites
(c) Chromatographic resolution
PepSep C18 columns are the preferred column in academic and core labs that are focused on speed, reproducibility and sensitivity. Easily connect columns with a simple twist on and twist off design for nanospray.
Spend less time swapping columns and more time on the important things, results. Amplify your experience when you combine PepSep consumables with timsTOF series instruments, powered by Bruker ProteoScape to enhance your ability to acquire data at PASEF® speeds.
Ultra-high sensitivity and reproducibility at PASEF® speeds
Exceed sample throughput limits at PASEF® speeds with high 4D-sensitivity
Maximize performance to accurately analyze 1000s of samples
Choosing the right column is difficult. A make-or-break decision that may increase protein and peptide identifications. The PepSep ULTRA (25 cm x 75 µm x 1.5 µm) C18 HPLC columns enable you to analyze a higher number of proteins and peptides from routine to restricted sample types.
When paired with the timsTOF SCP, exceed your limits of reproducibility, with maximum sensitivity even at PASEF® speed.
Longer gradients with higher sensitivity and proteome depth are now possible. Identify more than 2000 proteins from 500 pg of cell digest and 1500 proteins identified from 250 pg easily as shown on the right. Discover true sensitivity and uncover proteomic patterns, one cell at a time to take your single cell proteomics to the next level with PepSep ULTRA.
Core labs and academic labs want a single column solution for all applications to achieve deeper coverage, higher throughput, and better identification.
PepSep XTREME (25 cm x 150 µm x 1.5 µm) will help increase instrument performance, allowing scientists the liberty to have both sample throughput at PASEF® speeds and high 4D-sensitivity to dive deeper into the unknown and open the potential of the plasma proteome.
Rather than deciding between a shorter gradient and high throughput, PepSep XTREME C18 HPLC columns offer you the best of both worlds. It’s time to improve your proteomics analysis with better coverage, throughput, sensitivity, and reproducibility. Pair the PepSep XTREME with the timsTOF Pro 2 to find unprecedented proteomics depths — more than 5500 protein groups, 42,000 peptides in a 30 min gradient.
Scientists spend time fine tuning their methods to achieve broader coverage and higher throughput when mining plasma proteomes.
The PepSep MAX (10 cm x 150 µm x 1.5 µm) increases instrument performance to help drive your analysis. When combined with the timsTOF HT, immerse yourself in 4D-Proteomics™, so you can accurately analyze 1000s of samples with high throughput at PASEF® speed.
The proteomics landscape requires high throughput to capture meaningful rich data. PepSep MAX columns help you get there. It's time to improve your HPLCs capabilities.
Identify, characterize and quantify peptides and proteins. Get excellent reproducibility, while outlasting leading columns up to 2x longer.
"At first, we were just using PepSep columns on our Bruker timsTOF Pro mass spectrometer. But we have been so impressed with their performance and affordable price point that we now use them across all instruments in our lab.
These columns really hit a sweet spot of top-notch chromatography but with minimal back pressure. When compared to traditional nanospray columns, this allows us to use higher flow rates and minimize LC overhead time. This has been crucial for throughput, particularly when thousands of samples using shorter gradients."
Ready to use pulled fused silica emitter without the hassle. Simple plug and play design. Spend less time worrying about tip setup with a static tip ready to use out of the box.
Available in two internal identifications for high throughput, plasma, or single cell proteomics applications.
For Research Use Only. Not for use in clinical diagnostic procedures.