Single-molecule localization microscopy (SMLM) provides the ability to image biological processes well below the diffraction limit of light. Commercial as well as homemade systems for SMLM typically utilize TIRF based illumination which limits the depth of imaging to a few microns from the cover slip. This restricts the range of detection to only part of cultured adherent cells, and makes imaging of cell colonies, tissues, or small organisms impractical.
The Vutara SMLM system is not constrained by this limitation. Utilizing biplane detection along with widefield illumination, the Vutara is capable of imaging throughout the entire axial dimension of cultured adherent cells, as well as tissue and small organisms. This webinar will describe the technical details of Vutara, as well as providing application examples demonstrating the use of the Vutara for SMLM in cultured cells, tissues and small organisms for various SMLM techniques such as STORM and DNA PAINT.
Dr. Lauren Gagnon
Vutara Applications Scientist, Bruker