Recent developments in high-speed AFM imaging (>10 fps) and the development of advanced imaging and quantitative property mapping modes have greatly expanded our ability to characterize kinetic and dynamic processes as well as very soft, very small, and fragile structures like single molecules in liquid.
The NanoWizard® ULTRA Speed 2 with the Vortis™ controller can be coupled to a super-resolution STED instrument. This setup leverages the advantages of both techniques to enable true correlative microscopy and non-invasive examination of specific molecules or features carrying immunochemical information.
In this application note, we exhibit the capability of the NanoWizard ULTRA Speed instrument to non-invasively scan DNA nanostructures at 400 lines/s in closed-loop mode. We demonstrate that the instrument can: