S2 PICOFOX, TXRF Spectrometer, Trace Analysis

Sample Preparation of Plants, Food, Nutrients and Similar

Quantitative procedure without digestion

  • Grind dried sample in a mortar or ball mill
  • Weigh about 50 mg in a tube and add 2.5 ml 1 Vol.-% aqueous Triton X-100 solution
  • Add 10 μl IS solution (Ga, 1 g/L, end conc. 200 mg/kg) and homogenize in an ultrasonic bath for 15 min
  • Pipette 10 μl on a siliconized sample carrier and dry by vacuum

Procedure for micro samples

  • Weigh the sample in a micro cup with an ultra-balance and note the exact weight
  • Add 500 μl Triton X-100 and 10 μl internal standard (Ga, 100 mg/L, end conc. 20 mg/kg)
  • Homogenize, load carrier with 10 μl suspension

Remarks

  • Triton X-100 is a common detergent, which is used to adjust the viscosity of aqueous solutions
  • For matrix-rich samples the calibration for light elements (Z < 20) should be optimized

TXRF benefits

  • Preparation of a suspension after grinding avoids time-consuming digestion
  • Easy analysis of wet and lubricious samples
s2_picofox_peaches.jpg
Samples: peaches
s2_picofox_LLD_plant.jpg
Detection limits for plant standards prepared
as suspension