NMR Instruments

DNP-NMR

The world’s first commercially available dynamic nuclear polarization (DNP-NMR) systems for enhanced sensitivity of solid-state NMR. All Bruker DNP-NMR spectrometers enable extended solid-state NMR experiments with unsurpassed sensitivity for exciting new applications in biomolecular research, material science, and pharmaceuticals.

Making the

invisible visible

Highlights

400

Signal enhancements range from a factor of 20 to 400, driven by microwave irradiation to transfer polarization from unpaired electron spins to nuclear spins.

263

GHz

A 263 GHz klystron is also available for DNP experiments at 400 MHz, delivering top-notch signal enhancements at a lower cost and with reduced infrastructure requirements.

200

Polarization enhancement yields a factor of up to 200 gain in sensitivity for solid-state NMR

Solid-State DNP at 263-593 GHz

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DNP with Fast MAS: Rapid Structural Characterization

25 kHz MAS improves the resolution of DNP spectra, enables long-range inter-residue polarization transfers, and is helpful for extending assignments. DNP experiments were performed on Pf1 bacteriophage with non-uniform sampling (NUS) for rapid acquisition of multidimensional experiments.

Improved sensitivity from DNP allows the characterization of expansin protein binding to plant cell walls

DNP experiments allow rapid detection of ~1% U-13C,15N expansin mixed with plant cell walls. A REDOR filter selects only signals from the expansin 13C signals and spin diffusion mixing following the REDOR filter reveals correlations between expansin and the cell-wall polysaccharides. Comparison of the wild-type protein with two mutants indicate that site-specific cellulose binding is correlated with strong wall-loosening activity.

DNP-enhanced NMR in biological solids

A wide range of biological samples have been successfully enhanced on the Bruker DNP-NMR spectrometer including small peptides, soluble proteins, membrane proteins, and large biological complexes.

Material Science

DNP NMR allows the characterization, at the molecular level, of hybrid organic silica material. These materials are key compounds for applications in catalysis, drug delivery, separation and purification devices.

Gyrotron Microwave Source

The gyrotron includes a sealed custom-designed gyrotron tube, superconducting magnet, and control system, all designed to provide high stability, reliability, and ease of operation. Beam propagation to the sample is ensured by high microwave beam quality and corrugated waveguide. The sample is polarized in-situ in a low-temperature NMR MAS probe.

Gyrotron Microwave Source

¹H NMR Frequency	WB NMR Magnet	Gyrotron Frequency	Gyrotron Magnet
400 MHz	400/89 Ascend DNP	263 GHz	4.8 T cryogen-free
600 MHz	600/89 Ascend DNP	395 GHz	7.2 T cryogen-free
800 MHz	800/89 USP RS	527 GHz	9.7 T cryogen-free

1.3 and 1.9 mm DNP MAS Probe

DNP experiments are performed at low temperature (100 K) for efficient transfer of polarization from electron spins to nuclear spins. Until recently, the spinning frequency at 100 K was limited to 15 kHz with a 3.2 mm rotor. With the introduction of the Bruker 1.3 and 1.9 mm low-temperature MAS (LTMAS) probe, DNP experiments can now be performed at up to 40 kHz (1.3) and 24 kHz (1.9) MAS MAS frequency for enhanced spectral resolution. The fast MAS probes include pneumatic insert/eject capability at ambient and cold temperature. The ability to change samples while the probe remains cold is critical for optimal use of experiment time. The probe is available with HCN, HXY, or HX NMR circuits.